{"product_id":"human-lcn2-shrna-lentivirus-bhv19400130","title":"Human LCN2 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eLipocalin-2 (LCN2), also known as NGAL, is a small secreted glycoprotein of the lipocalin family that sequesters iron-loaded bacterial siderophores, contributing to innate antibacterial immunity by limiting iron availability. Beyond this role, LCN2 participates in iron transport, cellular stress responses, and inflammation, and acts as an acute-phase protein induced during infection and tissue injury. LCN2 expression is elevated in inflammatory conditions, kidney injury, and metabolic disease, and it is upregulated in several cancers, where it has been linked to tumor growth, survival, and invasion. These roles make LCN2 a target of interest in inflammation, metabolism, and cancer research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Human LCN2 shRNA Lentivirus delivers validated short hairpin RNA targeting LCN2 from a third-generation, self-inactivating lentiviral backbone. shRNA expression is driven by a U6 promoter, with a co-expressed fluorescent reporter (GFP or RFP) and antibiotic selection marker. VSV-G pseudotyping enables broad tropism across primary, suspension, and cryopreserved cells, and each shRNA is validated for at least 70% LCN2 knockdown by a fluorescence-based method.\u003c\/p\u003e\n\u003cp\u003eHigh-titer particles are ultra-purified by PEG precipitation and sucrose gradient centrifugation. A shRNA set option provides a mix of two independent validated shRNAs plus a scrambled control. Applications include loss-of-function studies of iron homeostasis, inflammation, and tumor progression.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting LCN2 (NCBI Accession: NM_005564) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251618079085,"sku":"LSV-0013-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311264653677,"sku":"LSV-0013-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311264686445,"sku":"LSV-0013-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311264719213,"sku":"LSV-0013-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311264751981,"sku":"LSV-0013-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311264784749,"sku":"LSV-0013-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311264817517,"sku":"LSV-0013-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311264850285,"sku":"LSV-0013-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311264883053,"sku":"LSV-0013-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311264915821,"sku":"LSV-0013-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0013-h-LCN2-sh-Fig1.jpg?v=1776934143","url":"https:\/\/www.ebiohippo.com\/products\/human-lcn2-shrna-lentivirus-bhv19400130","provider":"BioHippo","version":"1.0","type":"link"}