{"product_id":"human-mast-cell-line-lad2-bhc10902116","title":"Human Mast Cell Line (LAD2) ; \u003cspan style=\"color: #ef6331;\"\u003eCapable of histamine release (degranulation)\u003c\/span\u003e","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe LAD2 human mast cell line originates from bone marrow aspirates of a patient with mast cell sarcoma\/leukemia. These cells exhibit ultrastructural characteristics of human mast cells and express FcεRI, along with markers such as CD4, 9, 13, 14, 22, 31, 32, 45, 64, 71, 103, 117, 132, CXCR4 (CD184), and CCR5 (CD195).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Human Mast Cell Line (LAD2) ; \u003cspan style=\"color: #ef6331;\"\u003eCapable of histamine release (degranulation)\u003c\/span\u003e is supplied as a tumor cell line derived from Human bone marrow.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Suspension; Rounded, granulated; some large aggregates at higher densities\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Note: understanding the recommended culture conditions and expected behaviour of this cell line are critical for establishing successful cultures. PriGrow X Series Medium for T8157 (TM8157) + 100 ng\/ml human Stem Cell Factor (Z100815) + 2 mM L-glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Replace half of the medium with an equal volume of fresh medium, weekly. Do not allow cells to become over-confluent or grow past a density of 1x106 cells\/ml. Note: it is normal to observe cell death in the first two weeks after thawing. Allow cells to recover under the recommended culture conditons. Cells are slow-growing and extremely sensitive to mycoplasma contamination; routine testing should be performed. *Cells may lose functionality as a result of continuous culture. Degranulation assays must be conducted every 2 months to test functionality. For optimal degranulation, 1 million LAD2 and LADR cells should be cultured in hSCF-free media for 4 hours prior to sensitization. The cells should then be sensitized with human IgE (Y70000), followed by IgE crosslinking (Y79000) to stimulate degranulation before performing the Mast Granulation Assay (G7800). For more details refer to Mast Cell Preparation for Granulation Assay - Guideline.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eAdditionally, they contain intracytoplasmic histamine, tryptase, and chymase. Notably, LAD2 cells do not carry activating mutations at codon 816 of the c-kit gene. Similar to LAD1, LAD2 cells release β-hexosaminidase upon FcεRI or FcϒRI aggregation.The availability of the LAD2 mast cell line provides a unique opportunity to study the biology of human mast cells. Over time, it has become the most widely used and recognized gold-standard mast cell line for allergy and mastocytosis research, as well as a crucial tool in pharmaceutical development. Donor\/background information provided for this product: Male, 44, Mastocytosis.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Note: understanding the recommended culture conditions and expected behaviour of this cell line are critical for establishing successful cultures. PriGrow X Series Medium for T8157 (TM8157) + 100 ng\/ml human Stem Cell Factor (Z100815) + 2 mM L-glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Replace half of the medium with an equal volume of fresh medium, weekly. Do not allow cells to become over-confluent or grow past a density of 1x106 cells\/ml. Note: it is normal to observe cell death in the first two weeks after thawing. Allow cells to recover under the recommended culture conditons. Cells are slow-growing and extremely sensitive to mycoplasma contamination; routine testing should be performed. *Cells may lose functionality as a result of continuous culture. Degranulation assays must be conducted every 2 months to test functionality. For optimal degranulation, 1 million LAD2 and LADR cells should be cultured in hSCF-free media for 4 hours prior to sensitization. The cells should then be sensitized with human IgE (Y70000), followed by IgE crosslinking (Y79000) to stimulate degranulation before performing the Mast Granulation Assay (G7800). For more details refer to Mast Cell Preparation for Granulation Assay - Guideline.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/ml):\u003c\/strong\u003e 500,000-800,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180541796717,"sku":"T8157","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/fr5RQt8gaoF0xOkhVlDyKntjIxtvzMiACN30Ao1P.png?v=1774957856","url":"https:\/\/www.ebiohippo.com\/products\/human-mast-cell-line-lad2-bhc10902116","provider":"BioHippo","version":"1.0","type":"link"}