{"product_id":"human-mast-cell-line-ladr-bhc10902114","title":"Human Mast Cell Line (LADR) ; \u003cspan style=\"color: #ef6331;\"\u003eCapable of histamine release (degranulation)\u003c\/span\u003e","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eLADR cells were derived from CD34⁺ progenitor cells obtained via bone marrow aspiration from a patient with aggressive mastocytosis, despite the absence of identifiable KIT mutations.LADR cells exhibited log-fold increases in FcεRI\/CD117 expression and expressed CD13, CD33, CD34, CD63, CD117, CD123, CD133, CD184, CD193, and CD195. In contrast, previously established LAD2 cells expressed CD33, CD34, CD63, CD117, CD133, and CD193 but lacked CD13, CD123, CD184, and CD195.Morphologically, LADR cells were larger and more granulated, as observed through Wright–Giemsa staining and flow cytometry.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Human Mast Cell Line (LADR) ; \u003cspan style=\"color: #ef6331;\"\u003eCapable of histamine release (degranulation)\u003c\/span\u003e is supplied as a tumor cell line derived from Human bone marrow.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Suspension; Rounded, granulated; some large aggregates at higher densities\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Note: understanding the recommended culture conditions and expected behaviour of this cell line are critical for establishing a successful culture. PriGrow X Series Medium for T8156 (TM8156) + 100 ng\/ml human Stem Cell Factor (Z100815) + 2 mM L-glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: Thaw cells in a T75; remove debris during the first week post-thaw by skimming, where possible. It is normal to observe cell death in the first two-three weeks after thawing. Allow cells to recover under the recommended culture conditons. Replace half of the medium with an equal volume of fresh medium, weekly. Cells are slow-growing and extremely sensitive to mycoplasma contamination; routine testing should be performed. Do not allow cells to become over-confluent or grow past a density of 1x106 cells\/ml. *Cells may lose functionality as a result of continuous culture. Degranulation assays must be conducted every 2 months to test functionality. For optimal degranulation, 1 million LAD2 and LADR cells should be cultured in hSCF-free media for 4 hours prior to sensitization. The cells should then be sensitized with human IgE (Y70000), followed by IgE crosslinking (Y79000) to stimulate degranulation before performing the Mast Granulation Assay (G7800). For more details refer to Mast Cell Preparation for Granulation Assay - Guideline.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eTheir doubling time was approximately 4 weeks, significantly slower than LAD2 cells, which doubled every 2 weeks.Thus, LADR cells represent a distinct mast cell subline with slower proliferation, more advanced differentiation, increased FcεRI\/CD117 and tryptase expression, and a unique gene expression profile, making them a valuable model for studying mast cell development and mastocytosis. Donor\/background information provided for this product: Male, 44, Mastocytosis.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Note: understanding the recommended culture conditions and expected behaviour of this cell line are critical for establishing a successful culture. PriGrow X Series Medium for T8156 (TM8156) + 100 ng\/ml human Stem Cell Factor (Z100815) + 2 mM L-glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: Thaw cells in a T75; remove debris during the first week post-thaw by skimming, where possible. It is normal to observe cell death in the first two-three weeks after thawing. Allow cells to recover under the recommended culture conditons. Replace half of the medium with an equal volume of fresh medium, weekly. Cells are slow-growing and extremely sensitive to mycoplasma contamination; routine testing should be performed. Do not allow cells to become over-confluent or grow past a density of 1x106 cells\/ml. *Cells may lose functionality as a result of continuous culture. Degranulation assays must be conducted every 2 months to test functionality. For optimal degranulation, 1 million LAD2 and LADR cells should be cultured in hSCF-free media for 4 hours prior to sensitization. The cells should then be sensitized with human IgE (Y70000), followed by IgE crosslinking (Y79000) to stimulate degranulation before performing the Mast Granulation Assay (G7800). For more details refer to Mast Cell Preparation for Granulation Assay - Guideline.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/ml):\u003c\/strong\u003e 500,000-1,000,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180541010285,"sku":"T8156","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TQyOLm1fTcko5aZktJcqQlpjwmph1JUgH41CenZt.png?v=1774957856","url":"https:\/\/www.ebiohippo.com\/products\/human-mast-cell-line-ladr-bhc10902114","provider":"BioHippo","version":"1.0","type":"link"}