{"product_id":"human-mucoepidermoid-carcinoma-cell-line-um-hmc-3a-bhc10902098","title":"Human Mucoepidermoid Carcinoma Cell Line (UM-HMC-3A)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe UM-HMC series, encompassing UM-HMC-1, UM-HMC-3B, and UM-HMC-3A, are pioneering cell lines derived from human salivary mucoepidermoid carcinomas, pivotal for advancing research in oncology and cellular biology. Originating from a primary tumor (UM-HMC-1) from a male patient, and recurrence and lymph node metastasis from a female patient (UM-HMC-3A and UM-HMC-3B, respectively), these cell lines have been instrumental in characterizing the tumorigenic potential and cancer stem cell properties within salivary gland carcinomas.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Human Mucoepidermoid Carcinoma Cell Line (UM-HMC-3A) is supplied as a tumor cell line derived from Human salivary gland.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 10% FBS(Regular*) + 1% L-glutamine (G275) + 400 ng\/ml hydrocortisone (TM066) + 20 ng\/ml rhEGF (Z100139) + 5 ug\/ml recombinant human Insulin (Z101065) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: re-suspend cells well at each stage (thawing, post-centrifugation etc.) as cells have a tendency to clump. Freeze-thaw viability for this cell line varies. For best results, seed thawed cells, collect floating cells the next day, centrifuge and re-plate into a new flask. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in neurobiology, differentiation, and cell signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eStudies utilizing these cell lines have shed light on critical aspects of cancer biology, including the role of ALDH\/CD44 in identifying tumorigenic cancer stem cells, the necessity of mTOR signaling for the survival of these cancer stem cells, and the influence of p53 in regulating Bmi-1-driven self-renewal and stemness. As such, the UM-HMC series represents a valuable resource for researchers aiming to explore the molecular mechanisms underlying salivary gland cancer progression, metastasis, and treatment resistance, thereby offering potential pathways for developing targeted therapies.Complete list of cell lines from this panel: Cat. T8160 - Human Mucoepidermoid Carcinoma Cell Line (UM-HMC-3A)Cat. T8161 - Human Mucoepidermoid Carcinoma Cell Line (UM-HMC-3B) Cat. T8173 - Human Mucoepidermoid Carcinoma Cell Line (UM-HMC-1) Donor\/background information provided for this product: Female, 73, Caucasian, , Left hard palate (local recurrence = 3A cell line) and lymph node metastasis (=3B cell line), T4BN, stage IVb, intermediate grade, + for both perineural and angio-lymphatic invasion..\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNeurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.\u003c\/li\u003e\n\u003cli\u003eCell-based assays that compare experimental perturbations across defined media and substrate conditions.\u003c\/li\u003e\n\u003cli\u003ePhenotype tracking using morphology, marker expression, or reporter output where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 10% FBS(Regular*) + 1% L-glutamine (G275) + 400 ng\/ml hydrocortisone (TM066) + 20 ng\/ml rhEGF (Z100139) + 5 ug\/ml recombinant human Insulin (Z101065) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: re-suspend cells well at each stage (thawing, post-centrifugation etc.) as cells have a tendency to clump. Freeze-thaw viability for this cell line varies. For best results, seed thawed cells, collect floating cells the next day, centrifuge and re-plate into a new flask. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:10 to 1:4\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180540649837,"sku":"T8160","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/z6sDNCZTqAqvECyhikQKSGMtOZ9o7EKXieOh1joD.png?v=1774957854","url":"https:\/\/www.ebiohippo.com\/products\/human-mucoepidermoid-carcinoma-cell-line-um-hmc-3a-bhc10902098","provider":"BioHippo","version":"1.0","type":"link"}