{"product_id":"human-myotonic-dystrophy-type-2-fibroblast-cells-dm2-a-bhc10923194","title":"Human Myotonic Dystrophy Type 2 Fibroblast Cells (DM2-A)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis novel myotonic dystrophy type 2 (DM2) fibroblast cell line is useful for investigations regarding type 2 myotonic dystrophy. The product is supplied as frozen cells.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological source:\u003c\/strong\u003e Human (H. sapiens)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue origin:\u003c\/strong\u003e Skin\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, fibroblast\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Frozen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding guidance:\u003c\/strong\u003e 200,000 - 1,000,000 cells\/cm²\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e II\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCulture context:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eFibroblast-based disease models are commonly used to study cell-autonomous phenotypes, stress responses, extracellular matrix remodeling, and pathway changes associated with inherited disorders.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePatient-derived primary cells are used to connect genotype to measurable cellular phenotypes in vitro.\u003c\/li\u003e\n\u003cli\u003eComparative studies often evaluate disease-relevant stress, signaling, and transcriptomic changes across matched controls.\u003c\/li\u003e\n\u003cli\u003eCryopreserved donor-derived fibroblasts support repeatable expansion and banking for longitudinal assay development.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eCompare disease-associated phenotypes with control fibroblasts under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eMeasure transcriptional, metabolic, or signaling changes following perturbation.\u003c\/li\u003e\n\u003cli\u003eUse as a donor-derived cell source for reprogramming, co-culture, or mechanistic follow-up studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eProduct-specific data supplied for this listing\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 20% FBS + 1% Non-Essental Amino Acids (TM068) + 1% HEPES (TM058) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWarranty:\u003c\/strong\u003e abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period”.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 200,000 - 1,000,000\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.\u003c\/li\u003e\n\u003cli\u003eAttachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.\u003c\/li\u003e\n\u003cli\u003eCryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078) or, if serum is preferred, Cryopreservation Medium (TM024).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC10923194\u003c\/p\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"5x10\u003csup\u003e5\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53195757584749,"sku":"T4155","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/g1IJFrr251QaAW4M8usoiBVyT6gTRR4MrUdsyVwm.jpg?v=1775322489","url":"https:\/\/www.ebiohippo.com\/products\/human-myotonic-dystrophy-type-2-fibroblast-cells-dm2-a-bhc10923194","provider":"BioHippo","version":"1.0","type":"link"}