{"product_id":"human-n-acetylglucosaminyl-phosphatidylinositol-de-n-acetylase-pigl-elisa-kit-bhe13703427","title":"Human N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (PIGL) ELISA Kit","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (PIGL) ELISA Kit has high sensitivity and excellent specificity for detection of Human PIGL. No significant cross-reactivity or interference between Human PIGL and analogues was observed.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eGlycosylphosphatidylinositol (GPI) is used as a membrane anchor by many eukaryotic cell surface proteins. The first step in GPI biosynthesis involves the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to phosphatidylinositol (PI) . The second step is de-N-acetylation of GlcNAc-PI.\u003c\/p\u003e\u003cp\u003ePIGL encodes a deduced 252-amino acid protein that shares 77% identity with the rat protein. Using transfection into mammalian PIGL-deficient cells, Watanabe et al. (1999) demonstrated that S. cerevisiae Gpi12 is the ortholog of human and rat PIGL. Disruption of Gpi12 in yeast resulted in a lethal phenotype. Using purified, recombinant rat PIGL, Watanabe et al. (1999) demonstrated that PIGL has GlcNAc-PI de-N-acetylase activity in vitro, which is enhanced by metal ions.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"48 T","offer_id":53338624295277,"sku":"KTE61211-48T","price":339.0,"currency_code":"USD","in_stock":true},{"title":"96 T","offer_id":53338795016557,"sku":"KTE61211-96T","price":589.0,"currency_code":"USD","in_stock":true},{"title":"96 T×5","offer_id":53338795049325,"sku":"KTE61211-96TX5","price":2629.0,"currency_code":"USD","in_stock":true},{"title":"96 T×50","offer_id":53338795082093,"sku":"KTE61211-96TX50","price":24569.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/KTE61211.png?v=1778954734","url":"https:\/\/www.ebiohippo.com\/products\/human-n-acetylglucosaminyl-phosphatidylinositol-de-n-acetylase-pigl-elisa-kit-bhe13703427","provider":"BioHippo","version":"1.0","type":"link"}