{"product_id":"human-nkb-neurokinin-b-elisa-kit-bhe15200745","title":"Human NKB(Neurokinin B) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eNKB (Neurokinin B)\u003c\/strong\u003e is a neuroscience-related marker used to study neuronal\/glial biology, synaptic function, or neuroinflammatory processes (target dependent).\u003c\/p\u003e\u003cp\u003eChanges in marker concentrations can help relate functional outcomes (behavior, electrophysiology) to molecular changes in specific brain regions or fluids.\u003c\/p\u003e\u003cp\u003eQuantitative profiling is often most informative when combined with region-matched sampling and appropriate normalization\/controls.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eNKB (Neurokinin B)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eHuman\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NKB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NKB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NKB in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: Serum, plasma and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 12.5-800 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 3.19 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 2h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52964585668973,"sku":"ELK1801-96T","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52964585701741,"sku":"ELK1801-48T","price":416.0,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52964585734509,"sku":"ELK1801-96TX5","price":2531.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_37447acb-93ed-4d98-92b6-2e5b609b0b49.jpg?v=1771840871","url":"https:\/\/www.ebiohippo.com\/products\/human-nkb-neurokinin-b-elisa-kit-bhe15200745","provider":"BioHippo","version":"1.0","type":"link"}