{"product_id":"human-pancreatic-stellate-cells-hpsc-bhc18500067","title":"Human Pancreatic Stellate Cells (HPSC)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eHuman Pancreatic Stellate Cells (HPSC)\u003c\/strong\u003e is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Human NPC derived from Pancreas (Pancreatic Stellate) within the Digestive system.\u003c\/p\u003e\n\u003cp\u003eHuman Pancreatic Stellate Cells (HPSC) are the main fibroblastic cells of the pancreas. HPSC are responsible for the synthesis and the degradation of the extracellular matrix proteins that promote tissue repair. They are found adjacent to pancreatic acinar cells and around small pancreatic ducts and blood vessels. When HPSC are activated, they assume myofibroblast-like morphology and secrete excessive amounts of extracellular matrix proteins, which can lead to desmoplasia in chronic pancreatitis and adenocarcinoma [1,2] . Recent studies have shown that HPSC can also act as progenitor cells, immune cells, and intermediaries in exocrine pancreatic secretion [3] . Additionally, HPSC stimulate pancreatic cancer proliferation, inhibit apoptosis, and enhance angiogenesis [2] . HPSC are the ideal model for studying pancreatic tumor formation and they can be used to develop novel therapies for treatment of chronic pancreatitis and adenocarcinoma. iXCells Biotechnologies offers HPSC from human pancreas. HPSC are cryopreserved after purification and delivered frozen. Each vial contains \u0026gt;5 x 10 5 cells in 1 ml volume. HPSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPSC can be further expanded for no more than 3 passages in Stellate Cell Growth Medium ( Cat# MD-0014 ). Further expansion may decrease the purity. Figure1. Phase contrast image of Human Pancreatic Stellate Cells (HPSC) at DIV1.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCell identity:\u003c\/strong\u003e NPC (Primary Cells)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSource context:\u003c\/strong\u003e Pancreas; Pancreatic Stellate; Digestive\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e BSL-2 (follow your institution’s biosafety program and local regulations)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProduct-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eCells originating from the Digestive system are commonly studied to understand tissue-specific physiology, signaling, and responses to perturbations in controlled in vitro settings.\u003c\/p\u003e\u003cp\u003eAcross primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIncreasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.\u003c\/li\u003e\n  \u003cli\u003eAdoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.\u003c\/li\u003e\n  \u003cli\u003eIntegration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eProfile identity markers by flow cytometry or immunostaining in cultured cells\u003c\/li\u003e\n  \u003cli\u003eQuantify functional responses to defined stimuli relevant to the model system\u003c\/li\u003e\n  \u003cli\u003eCompare baseline phenotype across donors\/conditions using gene expression profiling\u003c\/li\u003e\n  \u003cli\u003eModel inflammatory or metabolic stress responses relevant to gastrointestinal tissues\u003c\/li\u003e\n  \u003cli\u003eScreen compounds or genetic perturbations for phenotype modulation using viability or imaging endpoints\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eDonor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.\u003c\/li\u003e\n  \u003cli\u003ePassage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.\u003c\/li\u003e\n  \u003cli\u003eContamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate negative\/positive controls for the readout (e.g., unstimulated controls, pathway agonists\/antagonists) to contextualize observed changes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- ATCC Animal Cell Culture Guide — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/animal-cell-culture-guide\n- Cell Line Authentication — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/cell-line-authentication\n- Biosafety in Microbiological and Biomedical Laboratories (BMBL) — U.S. HHS\/CDC\/NIH — https:\/\/www.cdc.gov\/labs\/BMBL.html\n- Mycoplasma contamination in cell culture — NCBI Bookshelf\/PMC — https:\/\/www.ncbi.nlm.nih.gov\/pmc\/\n- Primary cell culture considerations — Nature Methods — https:\/\/www.nature.com\/nmeth\/\n- Good cell culture practice guidelines — OECD\/ECVAM (concept) — https:\/\/www.oecd.org\/\n--\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC18500067\u003c\/p\u003e","brand":"iXCells Biotechnologies","offers":[{"title":"Cryopreserved \/ 0.5 million cells\/vial","offer_id":53197815447917,"sku":"10HU-211","price":1090.48,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10HU-211.png?v=1775378649","url":"https:\/\/www.ebiohippo.com\/products\/human-pancreatic-stellate-cells-hpsc-bhc18500067","provider":"BioHippo","version":"1.0","type":"link"}