{"product_id":"human-pbmcs-cryopreserved-bhc18500005","title":"Human PBMCs (Cryopreserved)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eHuman PBMCs (Cryopreserved)\u003c\/strong\u003e is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Human Immune Cells AND Hematopoietic Cells derived from Peripheral Blood (PBMCs) within the Blood system.\u003c\/p\u003e\n\u003cp\u003ePeripheral Blood Mononuclear Cells (PBMCs) is any blood cell with a single nucleus [1] . These blood cells are a critical component in human immune system to fight pathogen. PBMCs consist of lymphocytes (T, B and NK cells), monocytes and dendritic cells [2] . PBMCs are widely used in research fields including immunology, infectious diseases and hematological malignancies etc. iXCells Biotechnologies offers PBMCs from the human peripheral blood of healthy donors for research only. These PBMCs are purified from peripheral blood using Ficoll gradient centrifugation. We also provide fresh PBMCs. Please check HERE for more information.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCell identity:\u003c\/strong\u003e Immune Cells AND Hematopoietic Cells (Primary Cells)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSource context:\u003c\/strong\u003e Peripheral Blood; PBMCs; Blood\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e BSL-2 (follow your institution’s biosafety program and local regulations)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProduct-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eCells originating from the Blood system are commonly studied to understand tissue-specific physiology, signaling, and responses to perturbations in controlled in vitro settings.\u003c\/p\u003e\u003cp\u003eAcross primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIncreasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.\u003c\/li\u003e\n  \u003cli\u003eAdoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.\u003c\/li\u003e\n  \u003cli\u003eIntegration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.\u003c\/li\u003e\n  \u003cli\u003eExpansion of high-dimensional immune phenotyping and perturbation screens to map activation states and functional programs.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eProfile identity markers by flow cytometry or immunostaining in cultured cells\u003c\/li\u003e\n  \u003cli\u003eStimulate immune cells and quantify activation markers and cytokine release\u003c\/li\u003e\n  \u003cli\u003eQuantify functional responses to defined stimuli relevant to the model system\u003c\/li\u003e\n  \u003cli\u003eCompare baseline phenotype across donors\/conditions using gene expression profiling\u003c\/li\u003e\n  \u003cli\u003ePerform immune profiling by multiparameter flow cytometry to resolve major subsets\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eDonor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.\u003c\/li\u003e\n  \u003cli\u003ePassage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.\u003c\/li\u003e\n  \u003cli\u003eContamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate negative\/positive controls for the readout (e.g., unstimulated controls, pathway agonists\/antagonists) to contextualize observed changes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- ATCC Animal Cell Culture Guide — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/animal-cell-culture-guide\n- Cell Line Authentication — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/cell-line-authentication\n- Biosafety in Microbiological and Biomedical Laboratories (BMBL) — U.S. HHS\/CDC\/NIH — https:\/\/www.cdc.gov\/labs\/BMBL.html\n- Mycoplasma contamination in cell culture — NCBI Bookshelf\/PMC — https:\/\/www.ncbi.nlm.nih.gov\/pmc\/\n- Primary cell culture considerations — Nature Methods — https:\/\/www.nature.com\/nmeth\/\n- Good cell culture practice guidelines — OECD\/ECVAM (concept) — https:\/\/www.oecd.org\/\n--\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC18500005\u003c\/p\u003e","brand":"iXCells Biotechnologies","offers":[{"title":"Cryopreserved \/ 100 million cells\/vial","offer_id":53197811548525,"sku":"10HU-003-CR100M","price":587.6,"currency_code":"USD","in_stock":true},{"title":"Cryopreserved \/ 10 million cells\/vial","offer_id":53197820166509,"sku":"10HU-003-CR10M","price":146.64,"currency_code":"USD","in_stock":true},{"title":"Cryopreserved \/ 15 million cells\/vial","offer_id":53197820199277,"sku":"10HU-003-CR15M","price":185.12,"currency_code":"USD","in_stock":true},{"title":"Cryopreserved \/ 200 million cells\/vial","offer_id":53197820232045,"sku":"10HU-003-CR200M","price":741.52,"currency_code":"USD","in_stock":true},{"title":"Cryopreserved \/ 25 million cells\/vial","offer_id":53197820264813,"sku":"10HU-003-CR25M","price":271.44,"currency_code":"USD","in_stock":true},{"title":"Cryopreserved \/ 350 million cells\/vial","offer_id":53197820297581,"sku":"10HU-003-CR350M","price":929.76,"currency_code":"USD","in_stock":true},{"title":"Cryopreserved \/ 500 million cells\/vial","offer_id":53197820330349,"sku":"10HU-003-CR500M","price":1158.56,"currency_code":"USD","in_stock":true},{"title":"Cryopreserved \/ 50 million cells\/vial","offer_id":53197820363117,"sku":"10HU-003-CR50M","price":416.0,"currency_code":"USD","in_stock":true},{"title":"Cryopreserved \/ 5 million cells\/vial","offer_id":53197820395885,"sku":"10HU-003-CR5M","price":81.12,"currency_code":"USD","in_stock":true},{"title":"Pooled PBMC, cryopreserved \/ 10 million cells\/vial","offer_id":53197820428653,"sku":"10HU-003-P","price":204.88,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10HU-003-CR10M.png?v=1775378643","url":"https:\/\/www.ebiohippo.com\/products\/human-pbmcs-cryopreserved-bhc18500005","provider":"BioHippo","version":"1.0","type":"link"}