{"product_id":"human-peripheral-blood-cd3-positive-t-cells-pan-t-cells-bhc18500009","title":"Human Peripheral Blood CD3+ T Cells (Pan T Cells)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eHuman Peripheral Blood CD3+ T Cells (Pan T Cells)\u003c\/strong\u003e is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Human Immune Cells AND Hematopoietic Cells derived from Peripheral Blood (Peripheral Blood CD3+ T) within the Blood system.\u003c\/p\u003e\n\u003cp\u003eCD3 is a multimeric protein complex, which is composed of four distinct chains (CD3γ, CD3δ and two CD3ε). CD3 complex serves as a T-cell co-receptor that associates non-covalently with the T cell receptor (TCR) [1] . CD3 is expressed in almost all T cell lineages, which make it useful marker for T cells. T cells exhibit multiple functions including cell-mediated cytotoxicity, promotion of antibody production by B cells, and immune regulation. CD3+ T cells can be divided into several subsets. CD4+ helper T cell, CD8+ cytotoxic T cell and NK cells are the three major subsets. T cells have been widely used in research fields including immunology, infectious diseases ( Prednisone ), cancer therapy, etc. [2] iXCells Biotechnologies offers CD3+ Pan T Cells from the human peripheral blood of individual healthy donors using negative immunomagnetic selection. These cells are untouched by the separation process and ready for downstream usage. \u0026gt; 86% of the cells are CD3+ as shown by flow cytometric analysis. Figure 1. Flow cytometric analysis showed that \u0026gt;86% of the cells are CD3+.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCell identity:\u003c\/strong\u003e Immune Cells AND Hematopoietic Cells (Primary Cells)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSource context:\u003c\/strong\u003e Peripheral Blood; Peripheral Blood CD3+ T; Blood\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e BSL-2 (follow your institution’s biosafety program and local regulations)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProduct-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eCells originating from the Blood system are commonly studied to understand tissue-specific physiology, signaling, and responses to perturbations in controlled in vitro settings.\u003c\/p\u003e\u003cp\u003eAcross primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIncreasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.\u003c\/li\u003e\n  \u003cli\u003eAdoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.\u003c\/li\u003e\n  \u003cli\u003eIntegration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.\u003c\/li\u003e\n  \u003cli\u003eExpansion of high-dimensional immune phenotyping and perturbation screens to map activation states and functional programs.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eProfile identity markers by flow cytometry or immunostaining in cultured cells\u003c\/li\u003e\n  \u003cli\u003eStimulate immune cells and quantify activation markers and cytokine release\u003c\/li\u003e\n  \u003cli\u003eQuantify functional responses to defined stimuli relevant to the model system\u003c\/li\u003e\n  \u003cli\u003eCompare baseline phenotype across donors\/conditions using gene expression profiling\u003c\/li\u003e\n  \u003cli\u003ePerform immune profiling by multiparameter flow cytometry to resolve major subsets\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eDonor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.\u003c\/li\u003e\n  \u003cli\u003ePassage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.\u003c\/li\u003e\n  \u003cli\u003eContamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate negative\/positive controls for the readout (e.g., unstimulated controls, pathway agonists\/antagonists) to contextualize observed changes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- ATCC Animal Cell Culture Guide — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/animal-cell-culture-guide\n- Cell Line Authentication — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/cell-line-authentication\n- Biosafety in Microbiological and Biomedical Laboratories (BMBL) — U.S. HHS\/CDC\/NIH — https:\/\/www.cdc.gov\/labs\/BMBL.html\n- Mycoplasma contamination in cell culture — NCBI Bookshelf\/PMC — https:\/\/www.ncbi.nlm.nih.gov\/pmc\/\n- Primary cell culture considerations — Nature Methods — https:\/\/www.nature.com\/nmeth\/\n- Good cell culture practice guidelines — OECD\/ECVAM (concept) — https:\/\/www.oecd.org\/\n--\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC18500009\u003c\/p\u003e","brand":"iXCells Biotechnologies","offers":[{"title":"Cryopreserved \/ 20 million cells\/vial","offer_id":53197811450221,"sku":"10HU-009-20M","price":934.09,"currency_code":"USD","in_stock":true},{"title":"Cryopreserved \/ 40 million cells\/vial","offer_id":53197819904365,"sku":"10HU-009-40M","price":1565.02,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10HU-009-20M.png?v=1775378647","url":"https:\/\/www.ebiohippo.com\/products\/human-peripheral-blood-cd3-positive-t-cells-pan-t-cells-bhc18500009","provider":"BioHippo","version":"1.0","type":"link"}