{"product_id":"human-phosphopantothenate-cysteine-ligase-ppcs-elisa-kit-bhe11403034","title":"Human Phosphopantothenate-cysteine ligase (PPCS) ELISA Kit","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eSandwich ELISA Kit\u003c\/span\u003e\n\u003cp\u003eBiosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine.\nDaugherty et al. (2002) showed that recombinant PPCS functioned within the CoA synthetic pathway. PPCS used ATP for the activation of substrate in the ligation reaction 4 times more efficiently than CTP. Daugherty et al. (2002) verified the funct…\u003c\/p\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eThis assay employs a two-site sandwich ELISA to quantitate PPCS in samples. An antibody specific for PPCS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPCS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPCS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-e…\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eSandwich ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eSandwich ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003e0.156-10 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003c\/table\u003e\n\u003ch2\u003eStability \u0026amp; Storage\u003c\/h2\u003e\n\u003cp\u003eThe stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. \nThe loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Bio…\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO).\u003c\/strong\u003e Not intended for diagnostic or therapeutic use. Handle kit reagents according to standard laboratory safety guidelines.\u003c\/div\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eBrowse our complete selection of ELISA kits, antibodies, and assay reagents across species at BioHippo.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Wuhan Abebio Science Co Ltd","offers":[{"title":"48 T","offer_id":53336035164525,"sku":"AE26465HU-48T","price":325.0,"currency_code":"USD","in_stock":true},{"title":"96 T","offer_id":53336044470637,"sku":"AE26465HU-96T","price":610.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_4f5466ca-6dc6-4f39-b3d0-083e56029439.jpg?v=1778915260","url":"https:\/\/www.ebiohippo.com\/products\/human-phosphopantothenate-cysteine-ligase-ppcs-elisa-kit-bhe11403034","provider":"BioHippo","version":"1.0","type":"link"}