{"product_id":"human-primary-cd34-positive-blood-cells-bhc10900065","title":"Human Primary CD34+ Blood Cells","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHuman Primary CD34+ Blood Cells are hematopoietic stem and progenitor cells isolated from healthy adult donors. These cells provide a physiologically relevant in vitro model for studying hematopoiesis, blood cell differentiation, gene therapy, and immune system function. The product is supplied as frozen cells.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological source:\u003c\/strong\u003e Human (H. sapiens)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue origin:\u003c\/strong\u003e Blood (Immune)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Suspension, round\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Frozen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding guidance:\u003c\/strong\u003e 5000 cells\/ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e II\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCulture context:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) .\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003ePrimary cells provide a biologically relevant starting point for in vitro studies because they retain tissue-linked morphology, growth behavior, and donor-associated characteristics that are often reduced in immortalized systems.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary-cell models continue to be used when donor context, tissue specificity, and physiologic responsiveness are important.\u003c\/li\u003e\n\u003cli\u003eResearchers often combine primary cells with co-culture, matrix, or conditioned-media approaches to better reflect native microenvironments.\u003c\/li\u003e\n\u003cli\u003ePhenotypic characterization and careful tracking of culture conditions remain important for reproducible interpretation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUse in tissue-relevant in vitro assays where morphology and donor context matter.\u003c\/li\u003e\n\u003cli\u003eMeasure phenotype, marker expression, or response to defined perturbations over time.\u003c\/li\u003e\n\u003cli\u003eIncorporate into co-culture or screening workflows requiring primary-cell context.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eProduct-specific data supplied for this listing\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) . PriGrow X Series Medium (TM5402) + TM5402-CM + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: TM5402 must be supplemented with TM5402-CM (cytokine mix) for successful cell expansion. TM5402-CM is available as aliquots for either 100ml or 500ml of complete media.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:3 to 1:8\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/ml):\u003c\/strong\u003e 5000\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.\u003c\/li\u003e\n\u003cli\u003eAttachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.\u003c\/li\u003e\n\u003cli\u003eCryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC10900065\u003c\/p\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e5\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53195744215405,"sku":"T5402","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/tDhRbHUDMPXK0cbP4LzJFshjPILrqekfay7LdlQ0.png?v=1775322467","url":"https:\/\/www.ebiohippo.com\/products\/human-primary-cd34-positive-blood-cells-bhc10900065","provider":"BioHippo","version":"1.0","type":"link"}