{"product_id":"human-protein-dispatched-homolog-1-disp1-elisa-kit-bhe11405647","title":"Human Protein dispatched homolog 1 (DISP1) ELISA Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n\u003c\/style\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eThe pattern of cellular proliferation and differentiation that leads to normal development of embryonic structures often depends upon the localized production of secreted protein signals. Cells surrounding the source of a particular signal respond in a graded manner according to the effective concentration of the signal, and this response produces the pattern of cell types constituting the mature structure. A novel segment-polarity gene known as dispatched has been identified in Drosophila and its protein product is required for normal Hedgehog (Hh) signaling. DISP1 is one of two human homolog.\u003c\/p\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eThis assay employs a two-site sandwich ELISA to quantitate DISP1 in samples. An antibody specific for DISP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDISP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DISP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DISP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.\u003c\/p\u003e\n\u003ch2\u003ePerformance Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSensitivity\u003c\/th\u003e\n\u003ctd\u003eRequest Information\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003eRequest Information\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTotal Assay Time\u003c\/th\u003e\n\u003ctd\u003e3.5–5 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCompatible Sample Types\u003c\/th\u003e\n\u003ctd\u003eserum, plasma, cell culture supernatant, tissue homogenate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eColorimetric (TMB\/HRP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003e4°C (short-term); -20°C (long-term)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003e✓ Research-Grade Validation\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eResearch Use Only (RUO).\u003c\/strong\u003e This product is intended for research purposes only and is not approved for diagnostic, therapeutic, or clinical use.\u003c\/div\u003e\n\u003cp\u003eHandle reagents in accordance with institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for complete hazard and handling information. Contains components that may require special disposal procedures per local regulations.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Wuhan Abebio Science Co Ltd","offers":[{"title":"48 T","offer_id":53337748046189,"sku":"AE47200HU-48T","price":325.0,"currency_code":"USD","in_stock":true},{"title":"96 T","offer_id":53337913819501,"sku":"AE47200HU-96T","price":610.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_dbf70a8d-8b0e-468f-a140-14b16f31253c.jpg?v=1778953074","url":"https:\/\/www.ebiohippo.com\/products\/human-protein-dispatched-homolog-1-disp1-elisa-kit-bhe11405647","provider":"BioHippo","version":"1.0","type":"link"}