{"product_id":"human-pulmonary-fibroblasts-hpf-idiopathic-pulmonary-fibrosis-bhc18500276","title":"Human Pulmonary Fibroblasts (HPF, Idiopathic Pulmonary Fibrosis)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 14px;padding-left:22px}.bhp-desc li{margin-bottom:4px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-reg-item{display:inline-block;background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px;margin:3px 4px 3px 0}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}\u003c\/style\u003e\n\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eHuman Pulmonary Fibroblasts (HPF) isolated from lung tissue of patients with \u003cstrong\u003eidiopathic pulmonary fibrosis (IPF)\u003c\/strong\u003e. Fibroblasts play an important role in maintaining the structural integrity of connective tissue and in the synthesis of extracellular matrix proteins; in IPF this programme becomes dysregulated. Disease-derived fibroblasts provide a robust model for in vitro studies of IPF and related fibroblast diseases.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman (\u003cem\u003eHomo sapiens\u003c\/em\u003e)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTissue\u003c\/th\u003e\n\u003ctd\u003eLung\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDisease State\u003c\/th\u003e\n\u003ctd\u003eIdiopathic pulmonary fibrosis (IPF)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell Type\u003c\/th\u003e\n\u003ctd\u003eFibroblasts\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Format\u003c\/th\u003e\n\u003ctd\u003eCryopreserved\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003ctd\u003e0.5 x 10\u003csup\u003e6\u003c\/sup\u003e cells\/vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePassage at Cryopreservation\u003c\/th\u003e\n\u003ctd\u003eP2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eGrowth Properties\u003c\/th\u003e\n\u003ctd\u003eAdherent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecommended Medium\u003c\/th\u003e\n\u003ctd\u003eiXCells Fibroblast Growth Medium (MD-0011)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpansion Capacity\u003c\/th\u003e\n\u003ctd\u003eCan further expand for 10 population doublings\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eLiquid nitrogen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eHandling\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eThawing\u003c\/th\u003e\n\u003ctd\u003ePlace the vial in a 37 °C water bath for 1-2 minutes with gentle agitation, pipette into a tube with 5 mL medium, centrifuge at 1,000 rpm for 5 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSubculture\u003c\/th\u003e\n\u003ctd\u003eAt 80-90% confluence wash with DPBS, treat with 0.05% Trypsin-EDTA for 5 minutes at 37 °C, neutralise with 2-3 volumes of medium, centrifuge at 1,000 rpm for 5 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReseeding Density\u003c\/th\u003e\n\u003ctd\u003e5,000-10,000 cells\/cm\u003csup\u003e2\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpress \u003cstrong\u003efibronectin\u003c\/strong\u003e and \u003cstrong\u003evimentin\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eTested negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIPF disease modelling and anti-fibrotic compound screening.\u003c\/li\u003e\n\u003cli\u003eMyofibroblast differentiation and matrix-deposition assays.\u003c\/li\u003e\n\u003cli\u003eExtracellular matrix remodelling and contraction studies.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison against normal-donor pulmonary fibroblasts.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePair with normal-donor pulmonary fibroblasts; the disease phenotype is only interpretable against a matched healthy control.\u003c\/li\u003e\n\u003cli\u003eExpansion is limited to about 10 population doublings - the fibrotic phenotype drifts with extended passaging.\u003c\/li\u003e\n\u003cli\u003eRecord donor and lot with every experiment; IPF donor material is inherently variable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Intended Use\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eResearch Use Only.\u003c\/strong\u003e For Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Handle in accordance with good laboratory practice.\u003c\/div\u003e\n\u003cp\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eIntended Use\u003c\/strong\u003eResearch Use Only (RUO)\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eVendor\u003c\/strong\u003eiXCells Biotechnologies\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eCatalog No.\u003c\/strong\u003e10HU-240\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"iXCells Biotechnologies","offers":[{"title":"Cryopreserved \/ 0.5 million cells\/vial","offer_id":53197816758637,"sku":"10HU-240","price":1015.58,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/756c5baf-V01-1000px.jpg?v=1782157703","url":"https:\/\/www.ebiohippo.com\/products\/human-pulmonary-fibroblasts-hpf-idiopathic-pulmonary-fibrosis-bhc18500276","provider":"BioHippo","version":"1.0","type":"link"}