{"product_id":"human-puma-shrna-lentivirus-bhv19400154","title":"Human PUMA shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePUMA (p53 upregulated modulator of apoptosis), encoded by BBC3, is a BH3-only member of the BCL-2 protein family and a key effector of apoptosis. PUMA expression is induced by p53 following DNA damage and by p53-independent stimuli such as growth factor withdrawal and cellular stress. Once expressed, PUMA binds and inhibits anti-apoptotic BCL-2 proteins, freeing BAX and BAK to permeabilize the mitochondrial outer membrane and trigger caspase activation. Through this role, PUMA links diverse stress signals to programmed cell death and contributes to tumor suppression, making it an important target in cancer research, apoptosis studies, and investigations of treatment resistance.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Human PUMA shRNA Lentivirus delivers validated short hairpin RNA targeting BBC3 from a third-generation, self-inactivating lentiviral backbone, with shRNA expression driven by a U6 promoter and a co-expressed fluorescent reporter (GFP or RFP) and antibiotic selection marker. VSV-G pseudotyping supports broad tropism, including primary, suspension, and cryopreserved cells. Each shRNA is validated to achieve at least 70% PUMA knockdown using a fluorescence-based assay.\u003c\/p\u003e\n\u003cp\u003eThe high-titer particles are ultra-purified by PEG precipitation and sucrose gradient centrifugation. A shRNA set option provides a mix of two independent validated shRNAs plus a scrambled control. Applications include loss-of-function studies of apoptosis, tumor suppression, and stress responses, and generation of stable knockdown cell lines.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting BBC3 (NCBI Accession: NM_014417.5) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, RFP, GFP\/Luc, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251615359341,"sku":"LSV-0038-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311254135149,"sku":"LSV-0038-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311254167917,"sku":"LSV-0038-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311254200685,"sku":"LSV-0038-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311254233453,"sku":"LSV-0038-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311254266221,"sku":"LSV-0038-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311254298989,"sku":"LSV-0038-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311254331757,"sku":"LSV-0038-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311254364525,"sku":"LSV-0038-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311254397293,"sku":"LSV-0038-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0038-h-PUMA-sh-fig1.jpg?v=1776934210","url":"https:\/\/www.ebiohippo.com\/products\/human-puma-shrna-lentivirus-bhv19400154","provider":"BioHippo","version":"1.0","type":"link"}