{"product_id":"human-renal-proximal-tubular-epithelial-cells-hrptepc-bhc18500045","title":"Human Renal Proximal Tubular Epithelial Cells (HRPTEpC)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eHuman Renal Proximal Tubular Epithelial Cells (HRPTEpC)\u003c\/strong\u003e is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Human Epithelial Cells derived from Kidney (Renal Proximal Tubular) within the Urinary system.\u003c\/p\u003e\n\u003cp\u003eHuman renal proximal tubular epithelial cells (HRPTEpC) play a central role in renal physiology. They reabsorb the substances including the glucose and amino acids as well as control acid-base balance by the excretion of almost all the bicarbonate and the synthesis of ammonia. They are also involved in the excretion of metabolic end products. Furthermore, these cells are particularly sensitive to ischemic injury, and represent a primary target for xenobiotics, such as nephrotoxins (and their metabolites), whose effects can extend up to the kidney failure [1, 2] . HRPTEpC express IL-2R alpha and MHC class II antigens during inflammation, after renal transplantation, or in crescentic glomerulonephritis [3] . HRPTEpC culture provides a valuable in vitro model for studying the mechanisms of proximal tubular cell physiology and pathophysiology, as well as the potential mechanisms underlying nephrotoxins-induced renal toxicity. iXCells Biotechnologies provides high quality HRPTEpC, which are isolated from human kidneys and cryopreserved at P1, with ≥ 0.5 million cells in each vial. HRPTEpC express cytokeratin-18, -19, vimentin and ZO-1 [4] . They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HRPTEpC can be expanded for no more than 3 passages using Epithelial Cell Growth Medium ( Cat# MD-0041 ) under the conditions provided by iXCells Biotechnoliges. Further expansion may decrease the purity of the epithelial population. Figure 1. Human renal proximal tubular epithelial cells (HRPTEpC). (A) Phase contrast image of HPRTEpC. (B \u0026amp; C) Immunofluorescence staining with antibodies against ZO-1 (B) and Megalin (C).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCell identity:\u003c\/strong\u003e Epithelial Cells (Primary Cells)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSource context:\u003c\/strong\u003e Kidney; Renal Proximal Tubular; Urinary\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e BSL-2 (follow your institution’s biosafety program and local regulations)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProduct-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eEpithelial cells provide barrier and transport functions across tissues, coordinating innate defense, secretion, and repair responses in the face of environmental and inflammatory stressors.\u003c\/p\u003e\u003cp\u003eAcross primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIncreasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.\u003c\/li\u003e\n  \u003cli\u003eAdoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.\u003c\/li\u003e\n  \u003cli\u003eIntegration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eProfile identity markers by flow cytometry or immunostaining in cultured cells\u003c\/li\u003e\n  \u003cli\u003eQuantify functional responses to defined stimuli relevant to the model system\u003c\/li\u003e\n  \u003cli\u003eCompare baseline phenotype across donors\/conditions using gene expression profiling\u003c\/li\u003e\n  \u003cli\u003eAssess transporter or injury-response pathways relevant to renal\/urologic biology\u003c\/li\u003e\n  \u003cli\u003eScreen compounds or genetic perturbations for phenotype modulation using viability or imaging endpoints\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eDonor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.\u003c\/li\u003e\n  \u003cli\u003ePassage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.\u003c\/li\u003e\n  \u003cli\u003eContamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate negative\/positive controls for the readout (e.g., unstimulated controls, pathway agonists\/antagonists) to contextualize observed changes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- ATCC Animal Cell Culture Guide — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/animal-cell-culture-guide\n- Cell Line Authentication — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/cell-line-authentication\n- Biosafety in Microbiological and Biomedical Laboratories (BMBL) — U.S. HHS\/CDC\/NIH — https:\/\/www.cdc.gov\/labs\/BMBL.html\n- Mycoplasma contamination in cell culture — NCBI Bookshelf\/PMC — https:\/\/www.ncbi.nlm.nih.gov\/pmc\/\n- Primary cell culture considerations — Nature Methods — https:\/\/www.nature.com\/nmeth\/\n- Good cell culture practice guidelines — OECD\/ECVAM (concept) — https:\/\/www.oecd.org\/\n--\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC18500045\u003c\/p\u003e","brand":"iXCells Biotechnologies","offers":[{"title":"Cryopreserved \/ 0.5 million cells\/vial","offer_id":53197814104429,"sku":"10HU-112","price":793.76,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10HU-112.png?v=1775378646","url":"https:\/\/www.ebiohippo.com\/products\/human-renal-proximal-tubular-epithelial-cells-hrptepc-bhc18500045","provider":"BioHippo","version":"1.0","type":"link"}