{"product_id":"human-sertoli-cells-hserc-bhc18500052","title":"Human Sertoli Cells (HSerC)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eHuman Sertoli Cells (HSerC)\u003c\/strong\u003e is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Human Epithelial Cells derived from testes (Sertoli) within the Reproductive system.\u003c\/p\u003e\n\u003cp\u003eHuman Sertoli Cells (HSerC) are essential for testicular development, spermatogenesis, and formation of the blood-testis barrier [1, 2] . HSerC limit the passage of substances such as hormones and nutrients to the adluminal compartment of the seminiferous tubules [1] . In addition to forming the blood-testis barrier, HSerC also provide the main structural support for the seminiferous tubules and protect the germ cells from the immune system [1] . Aberrant HSerC proliferation can contribute to the development of male reproductive disorders such as testicular germ-cell cancer, cryptorchidism, hypospadias, and low sperm count [2] . HSerC proliferation is in part controlled by follicle-stimulating hormone (FSH) and thyroid hormone (TH), where FSH drives proliferation and TH promotes a more quiescent state [3] . Cultured HSerC are a useful in vitro model to better understand testicular dysgenesis syndrome and to develop treatments for male reproductive disorders. iXCells Biotechnologies provides high quality HSerC, which are isolated from human testis and cryopreserved at P1, with ≥ 1.5 million cells in each vial. HSerC express GATA-4 and Sox-9 and are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. They can further expand for no more than 3 passages in Sertoli Cell Growth Medium ( Cat# MD-0091 ) under the condition suggested by iXCells Biotechnologies. Further expansion may decrease the purity. Figure 1. Human Sertoli Cells (HSerC). The cells were recovered, and seeded at 10,000 cells\/cm 2 following iXCells’protocol. Phase contrast images were taken at the indicated time post recovery.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCell identity:\u003c\/strong\u003e Epithelial Cells (Primary Cells)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSource context:\u003c\/strong\u003e testes; Sertoli; Reproductive\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eDonor background:\u003c\/strong\u003e Gender: Male\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e BSL-2 (follow your institution’s biosafety program and local regulations)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProduct-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eEpithelial cells provide barrier and transport functions across tissues, coordinating innate defense, secretion, and repair responses in the face of environmental and inflammatory stressors.\u003c\/p\u003e\u003cp\u003eAcross primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIncreasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.\u003c\/li\u003e\n  \u003cli\u003eAdoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.\u003c\/li\u003e\n  \u003cli\u003eIntegration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eProfile identity markers by flow cytometry or immunostaining in cultured cells\u003c\/li\u003e\n  \u003cli\u003eQuantify functional responses to defined stimuli relevant to the model system\u003c\/li\u003e\n  \u003cli\u003eCompare baseline phenotype across donors\/conditions using gene expression profiling\u003c\/li\u003e\n  \u003cli\u003eModel hormone and cytokine signaling relevant to reproductive tissue physiology\u003c\/li\u003e\n  \u003cli\u003eScreen compounds or genetic perturbations for phenotype modulation using viability or imaging endpoints\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eDonor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.\u003c\/li\u003e\n  \u003cli\u003ePassage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.\u003c\/li\u003e\n  \u003cli\u003eContamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate negative\/positive controls for the readout (e.g., unstimulated controls, pathway agonists\/antagonists) to contextualize observed changes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- ATCC Animal Cell Culture Guide — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/animal-cell-culture-guide\n- Cell Line Authentication — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/cell-line-authentication\n- Biosafety in Microbiological and Biomedical Laboratories (BMBL) — U.S. HHS\/CDC\/NIH — https:\/\/www.cdc.gov\/labs\/BMBL.html\n- Mycoplasma contamination in cell culture — NCBI Bookshelf\/PMC — https:\/\/www.ncbi.nlm.nih.gov\/pmc\/\n- Primary cell culture considerations — Nature Methods — https:\/\/www.nature.com\/nmeth\/\n- Good cell culture practice guidelines — OECD\/ECVAM (concept) — https:\/\/www.oecd.org\/\n--\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC18500052\u003c\/p\u003e","brand":"iXCells Biotechnologies","offers":[{"title":"Cryopreserved \/ 1.5 million cells\/vial","offer_id":53197815906669,"sku":"10HU-149","price":1037.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10HU-149.png?v=1775378649","url":"https:\/\/www.ebiohippo.com\/products\/human-sertoli-cells-hserc-bhc18500052","provider":"BioHippo","version":"1.0","type":"link"}