{"product_id":"human-supar-soluble-urokinase-type-plasminogen-activator-receptor-sandwich-elisa-kit-stje0006654-bhe15506719","title":"Human suPAR (Soluble Urokinase-type Plasminogen Activator Receptor) Sandwich ELISA Kit (STJE0006654)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc ul{margin:0 0 14px;padding-left:22px}\n.bhp-desc li{margin-bottom:4px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600;margin:4px 6px 10px 0}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px;display:inline-block;margin:3px 4px 3px 0}\n.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin:0 6px 10px 0}\n\u003c\/style\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003esuPAR (STJE0006654)\u003c\/strong\u003e is a biomarker protein expressed in human tissues and biological fluids. Dysregulation of STJE0006654 has been implicated in cellular signaling and fundamental cell biology, making it a relevant biomarker for investigational studies.\u003c\/p\u003e\n\u003cp\u003eQuantitative measurement of STJE0006654 enables researchers to characterize its abundance in Serum, Plasma and correlate levels with experimental conditions. This kit recognizes Human suPAR in samples. No significant cross-reactivity or interference between Human suPAR and analogues was observed.\u003c\/p\u003e\n\u003cp\u003eThis sandwich ELISA kit provides a validated, reproducible format for STJE0006654 quantification across a defined standard curve range of 0.156~10ng\/mL. The assay supports experimental workflows in Cell Biology research.\u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cp\u003eEach kit contains sufficient reagents for one 96-well plate (approximately 40 samples in duplicate with standard curve):\u003c\/p\u003e\n\u003cp\u003ePre-coated 96-well microplate (capture antibody), HRP-conjugated detection antibody, recombinant standard protein, sample\/standard diluent, concentrated wash buffer, TMB substrate solution, stop solution, and product manual.\u003c\/p\u003e\n\u003cspan class=\"bhp-qc-badge\"\u003eValidated Lot\u003c\/span\u003e \u003cspan class=\"bhp-qc-badge\"\u003eColorimetric Readout\u003c\/span\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eSandwich ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003e0.156~10ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSensitivity (LLOD)\u003c\/th\u003e\n\u003ctd\u003e0.094ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidated Sample Types\u003c\/th\u003e\n\u003ctd\u003eSerum, Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTotal Assay Time\u003c\/th\u003e\n\u003ctd\u003e3.5 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eColorimetric (450 nm \/ 540–570 nm reference)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Format\u003c\/th\u003e\n\u003ctd\u003e96-well microplate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReactivity\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eAssay Procedure Summary\u003c\/h2\u003e\n\u003cp\u003eAdd samples and standards to pre-coated capture antibody wells and incubate. Wash, add HRP-conjugated detection antibody, and incubate. Wash again, add TMB substrate, and stop the reaction. Read absorbance at 450 nm (reference 540–570 nm). Interpolate sample concentrations from the standard curve using a 4-parameter logistic (4-PL) fit. Total protocol time: approximately 3.5 hours.\u003c\/p\u003e\n\u003cp\u003ePre-warm all kit components to room temperature (18–25 °C) for 30 minutes before starting. Run all samples in duplicate. Centrifuge samples at ≥1,000 × g before use to remove particulates.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Intended Use\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eResearch Use Only.\u003c\/strong\u003e For Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Handle in accordance with good laboratory practice.\u003c\/div\u003e\n\u003cp\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eIntended Use\u003c\/strong\u003eResearch Use Only (RUO)\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eVendor\u003c\/strong\u003eSt John's Laboratory Ltd\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eCatalog No.\u003c\/strong\u003eSTJE0006654\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003eHuman\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"St John's Laboratory Ltd","offers":[{"title":"24 T","offer_id":53335887905133,"sku":"STJE0006654-24","price":203.55,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":53335889576301,"sku":"STJE0006654-48","price":498.87,"currency_code":"USD","in_stock":true},{"title":"96 T","offer_id":53335889609069,"sku":"STJE0006654-96","price":600.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/human-supar-soluble-urokinase-type-plasminogen-activator-receptor-sandwich-elisa-kit-stje0006654-bhe15506719","provider":"BioHippo","version":"1.0","type":"link"}