{"product_id":"human-t-lymphoblast-stably-deoxycytidine-kinase-deficient-arac-8c-cell-line-bhc10901223","title":"Human T Lymphoblast Stably Deoxycytidine Kinase Deficient (AraC-8C) Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eHuman T lymphoblasts are susceptible to HIV infection, and past studies had focused on the anti-HIV drug, 2‘,3‘-dideoxycytidine (ddC) for treatment. ddC relies on the nucleoside transport system and studies have linked the deoxycytidine kinase is involved in drug metabolism. The Human T Lymphoblast Stably Deoxycytidine Kinase Deficient Cell Line (AraC-8C) is a mutant clonal derivative of the CCRF-CEM cell line, which is also hypoxanthine-guanine phosphoribosyltransferase-deficient.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Human T Lymphoblast Stably Deoxycytidine Kinase Deficient (AraC-8C) Cell Line is supplied as a tumor cell line derived from Human blood (immune).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Suspension, round lymphoblast-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 20% FBS + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eAraC-8D cells are able to grow in the presence of 5 µM arabinosylcytosine and are deficient in nucleoside transport activities making it resistant to ddC unlike the parental cells. Additionally, the cells exhibit resistance to Ara-C, commonly used anti-cancer chemotherapy drug. It is valuable for research relating to the functions of nucleoside transport and related pathways, and for anti-cancer chemotherapeutic analysis. Expression information reported for the model: Ara-C resistance.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 20% FBS + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWarranty:\u003c\/strong\u003e abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period”.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180521152877,"sku":"T3157","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TAMfhmS8LJbG18GQLV4LbMW4gKWRe1jgEYMyUdIQ.png?v=1774957805","url":"https:\/\/www.ebiohippo.com\/products\/human-t-lymphoblast-stably-deoxycytidine-kinase-deficient-arac-8c-cell-line-bhc10901223","provider":"BioHippo","version":"1.0","type":"link"}