{"product_id":"human-telomere-length-quantification-qpcr-assay-kit-bht15200048","title":"Human Telomere Length Quantification qPCR Assay Kit (Relative)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eTelomere Length Assay Kits (qPCR)\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eA telomere is a DNA sequence at the end of a eukaryotic chromosome composed of multiple repeating nucleotide elements (TTAGGG) in tandem. In addition to providing a buffer for non-transcribed DNA, it also protects chromosome ends from fusion and degradation, preserving chromosome stability and genetic integrity. Telomere length is determined initially by genetic and environmental factors and decreases over time. Studies have shown that telomere length is closely related to DNA repair, aging, apoptosis, and tumorigenesis. Therefore, accurate and repeatable measurement of telomere length is particularly important for researchers. This product mainly uses relative quantitative qPCR to directly compare the average telomere length of the sample, that is, the copy number ratio (T\/S) of the telomere repeat sequence (Tel) and the genome single copy gene (SCR) copy number (T\/S) is used as the telomere relative length. Single-copy gene primers (SCR) specifically recognize and amplify the 78 bp long region on human chromosome 11. The primer set in the kit has passed the test to ensure: (1) efficient and reliable quantification; (2) no non-specific amplification. Each set of primers has been verified by amplification curve efficiency (E\u0026gt;98%, R²\u0026gt;0.99), melting curve analysis and gel electrophoresis verification.\u003c\/p\u003e\n\u003cp\u003eThe assay is run in a 20 µL reaction with 0.5–5 ng of genomic DNA and a final primer concentration of 0.2 µM. Cycling is 95°C for 3 min, then 35–40 cycles of 95°C for 5 sec, 58°C for 30 sec and 72°C for 30 sec, followed by melting curve analysis. Relative telomere length is calculated by the comparative ∆∆Cq method as 2^(-∆∆Cq).\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eMethod\u003c\/th\u003e\n\u003ctd\u003eRelative quantitative qPCR with melting curve analysis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eTelomere repeat sequence (Tel) against a genomic single-copy reference gene (SCR)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReference gene region\u003c\/th\u003e\n\u003ctd\u003e78 bp region on human chromosome 11\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input\u003c\/th\u003e\n\u003ctd\u003e0.5–5 ng genomic DNA per 20 µL reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePrimer performance\u003c\/th\u003e\n\u003ctd\u003eE\u0026gt;98%, R²\u0026gt;0.99\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eResult principle\u003c\/th\u003e\n\u003ctd\u003eComparative ∆∆Cq; relative telomere length = 2^(-∆∆Cq)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eROX\u003c\/th\u003e\n\u003ctd\u003e50× ROX supplied separately; added according to the instrument\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReactions per kit\u003c\/th\u003e\n\u003ctd\u003e100 rxn or 500 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e100 rxn\u003c\/th\u003e\n\u003cth\u003e500 rxn\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2× Mix\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003ctd\u003e1.25 mL × 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTelomere Primer Mix (10 µM)\u003c\/td\u003e\n\u003ctd\u003e50 µL\u003c\/td\u003e\n\u003ctd\u003e100 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSingle Copy Reference Primer Mix (10 µM)\u003c\/td\u003e\n\u003ctd\u003e50 µL\u003c\/td\u003e\n\u003ctd\u003e100 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e50× ROX\u003c\/td\u003e\n\u003ctd\u003e250 µL\u003c\/td\u003e\n\u003ctd\u003e1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-free ddH₂O\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003ctd\u003e1.25 mL × 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRelative telomere length measurement in human samples\u003c\/li\u003e\n\u003cli\u003eComparison of average telomere length between samples\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eStored at -20°C and protected from light, with a shelf life of one year. Shipping: Ice pack or dry ice.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAt least three biological replicates are required.\u003c\/li\u003e\n\u003cli\u003eAvoid strong light during storage and reaction preparation.\u003c\/li\u003e\n\u003cli\u003eWear a lab coat and disposable operating gloves during the procedure.\u003c\/li\u003e\n\u003cli\u003eConfirm the amplification and melting curves after the reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEQ022\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200048\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"100 rxn","offer_id":53965513163117,"sku":"EQ022-100RXN","price":325.0,"currency_code":"USD","in_stock":true},{"title":"500 rxn","offer_id":53965515587949,"sku":"EQ022-500RXN","price":975.0,"currency_code":"USD","in_stock":true},{"title":"500 rxn x 5","offer_id":53965515620717,"sku":"EQ022-500RXNX5","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EQ022-a-1.jpg?v=1789419328","url":"https:\/\/www.ebiohippo.com\/products\/human-telomere-length-quantification-qpcr-assay-kit-bht15200048","provider":"BioHippo","version":"1.0","type":"link"}