{"product_id":"human-tnfrsf25-tumor-necrosis-factor-receptor-superfamily-member-25-elisa-kit-bhe15212747","title":"Human TNFRSF25(Tumor necrosis factor receptor superfamily member 25) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eTNFRSF25 (Tumor necrosis factor receptor superfamily member 25)\u003c\/strong\u003e is a TNF-family inflammatory mediator involved in cytokine cascades and cell fate signaling (survival\/apoptosis), depending on context.\u003c\/p\u003e\u003cp\u003eTNF-pathway markers are commonly monitored in innate immune activation and inflammatory disease models and can respond to endotoxin or immune-modulating treatments.\u003c\/p\u003e\u003cp\u003eProtein-level quantification helps compare cohorts and treatment effects, especially when transcriptional changes do not directly predict secreted protein abundance.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eTNFRSF25 (Tumor necrosis factor receptor superfamily member 25)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eHuman\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TNFRSF25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNFRSF25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TNFRSF25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TNFRSF25 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 234.38-15000 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 60.3 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52966449414509,"sku":"ELK10052-96T","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52966449447277,"sku":"ELK10052-48T","price":416.0,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52966449480045,"sku":"ELK10052-96TX5","price":2531.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_388a9128-1ad9-4cf4-b484-7d748e5392a2.jpg?v=1771848739","url":"https:\/\/www.ebiohippo.com\/products\/human-tnfrsf25-tumor-necrosis-factor-receptor-superfamily-member-25-elisa-kit-bhe15212747","provider":"BioHippo","version":"1.0","type":"link"}