{"product_id":"human-traf2-and-nck-interacting-protein-kinase-tnik-elisa-kit-bhe13702419","title":"Human TRAF2 and NCK-interacting protein kinase (TNIK) ELISA Kit","description":"\u003cp\u003e\u003cb\u003eFeatures \u0026amp; Benefits\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eHuman TRAF2 and NCK-interacting protein kinase (TNIK) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNIK. No significant cross-reactivity or interference between Human TNIK and analogues was observed.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eTNIK was autophosphorylated in a manner dependent upon lys54 in the ATP-binding pocket of its kinase domain. Immunoprecipitation analysis showed that epitope-tagged TNIK coprecipitated endogenous TRAF2 from human embryonic kidney cells. Mutation analysis revealed that the intermediate domain was sufficient for the interaction, although the GCK domain may contribute.\u003c\/p\u003e\u003cp\u003eThe intermediate domain was also sufficient for interaction with NCK. Cotransfection of TNIK with JNK2 (MAPK9) enhanced JNK2 kinase activity in a dose-dependent manner, and this effect was mediated by the GCK region of TNIK, but not the kinase domain. TNIK overexpression had no effect on ERK1 (MAPK3), p38 (MAPK14), or NF-kappa-B.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"48 T","offer_id":53338591822189,"sku":"KTE60200-48T","price":339.0,"currency_code":"USD","in_stock":true},{"title":"96 T","offer_id":53338695762285,"sku":"KTE60200-96T","price":589.0,"currency_code":"USD","in_stock":true},{"title":"96 T×5","offer_id":53338695795053,"sku":"KTE60200-96TX5","price":2629.0,"currency_code":"USD","in_stock":true},{"title":"96 T×50","offer_id":53338695827821,"sku":"KTE60200-96TX50","price":24569.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/KTE60200.png?v=1778954632","url":"https:\/\/www.ebiohippo.com\/products\/human-traf2-and-nck-interacting-protein-kinase-tnik-elisa-kit-bhe13702419","provider":"BioHippo","version":"1.0","type":"link"}