{"product_id":"human-villous-trophoblasts-hvt-bhc18500068","title":"Human Villous Trophoblasts (HVT)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eHuman Villous Trophoblasts (HVT)\u003c\/strong\u003e is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Human Epithelial Cells derived from placenta (Villous Trophoblasts) within the Reproductive system.\u003c\/p\u003e\n\u003cp\u003eThe trophoblast begins at the outer covering of the early blastocyst and provides the route of nourishment between the maternal endometrium and the developing embryo. The trophoblast adhesion to the uterine wall is the requisite first step of implantation and, subsequently, placentation. Human villous tryophoblasts (HVT) covering the villi of the placenta provide the surface for the exchange of oxygen and nutrients with the maternal circulation. They synthesize and release chorionic gonadotropin, placental lactogen and angiogenin [1] and express CXCR4, CCR5 and prolactin gene family [2, 3] . They acquire CCR1 as they differentiate to an invasive phenotype at the villous-anchoring sites [4] . The features of HVT, together with the recent establishment of trophoblast stem cells, make them an ideal genetic platform to study cell differentiation and organogenesis. iXCells Biotechnologies provides high quality HVT, which are isolated from human placental villi and cryopreserved at P1, with \u0026gt;0.5 million cells in each vial. HVT express alpha-and beta-HCG and are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fung. HVT can further expand no more than 3 passages in Trophoblast Growth Medium ( Cat# MD-0058 ) under the condition suggested by iXCells Biotechnologies. Further expansion may decrease the purity. Figure 1. Human Villous Trophoblasts (HVT) phase contrast image\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCell identity:\u003c\/strong\u003e Epithelial Cells (Primary Cells)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSource context:\u003c\/strong\u003e placenta; Villous Trophoblasts; Reproductive\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eDonor background:\u003c\/strong\u003e Gender: Female\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e BSL-2 (follow your institution’s biosafety program and local regulations)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProduct-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eEpithelial cells provide barrier and transport functions across tissues, coordinating innate defense, secretion, and repair responses in the face of environmental and inflammatory stressors.\u003c\/p\u003e\u003cp\u003eAcross primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIncreasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.\u003c\/li\u003e\n  \u003cli\u003eAdoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.\u003c\/li\u003e\n  \u003cli\u003eIntegration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eProfile identity markers by flow cytometry or immunostaining in cultured cells\u003c\/li\u003e\n  \u003cli\u003eQuantify functional responses to defined stimuli relevant to the model system\u003c\/li\u003e\n  \u003cli\u003eCompare baseline phenotype across donors\/conditions using gene expression profiling\u003c\/li\u003e\n  \u003cli\u003eModel hormone and cytokine signaling relevant to reproductive tissue physiology\u003c\/li\u003e\n  \u003cli\u003eScreen compounds or genetic perturbations for phenotype modulation using viability or imaging endpoints\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eDonor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.\u003c\/li\u003e\n  \u003cli\u003ePassage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.\u003c\/li\u003e\n  \u003cli\u003eContamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate negative\/positive controls for the readout (e.g., unstimulated controls, pathway agonists\/antagonists) to contextualize observed changes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- ATCC Animal Cell Culture Guide — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/animal-cell-culture-guide\n- Cell Line Authentication — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/cell-line-authentication\n- Biosafety in Microbiological and Biomedical Laboratories (BMBL) — U.S. HHS\/CDC\/NIH — https:\/\/www.cdc.gov\/labs\/BMBL.html\n- Mycoplasma contamination in cell culture — NCBI Bookshelf\/PMC — https:\/\/www.ncbi.nlm.nih.gov\/pmc\/\n- Primary cell culture considerations — Nature Methods — https:\/\/www.nature.com\/nmeth\/\n- Good cell culture practice guidelines — OECD\/ECVAM (concept) — https:\/\/www.oecd.org\/\n--\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC18500068\u003c\/p\u003e","brand":"iXCells Biotechnologies","offers":[{"title":"Cryopreserved \/ 0.5 million cells\/vial","offer_id":53197815349613,"sku":"10HU-214-0.5M","price":901.95,"currency_code":"USD","in_stock":true},{"title":"Cryopreserved \/ 1 million cells\/vial","offer_id":53197819969901,"sku":"10HU-214-1M","price":1192.25,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10HU-214-0.5M.png?v=1775378645","url":"https:\/\/www.ebiohippo.com\/products\/human-villous-trophoblasts-hvt-bhc18500068","provider":"BioHippo","version":"1.0","type":"link"}