{"product_id":"icam1-antibody-cd54-bha17135922","title":"ICAM1 Antibody \/ CD54","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eICAM1 Antibody \/ CD54 is a anti-ICAM1 Rabbit antibody Recombinant Rabbit Monoclonal clone 32I16 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Mouse. Reported localization: Cell surface.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ICAM1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32I16, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eICAM1 antibody detects Intercellular adhesion molecule 1, encoded by the ICAM1 gene. Intercellular adhesion molecule 1 is a cell surface glycoprotein belonging to the immunoglobulin superfamily. It is expressed on endothelial cells, epithelial cells, and immune cells, where it functions as an adhesion receptor binding to integrins such as LFA1 and Mac1. ICAM1 antibody provides researchers with a critical reagent for studying immune cell trafficking, inflammation, and signaling. The protein is upregulated in response to cytokines such as TNF alpha and IL1 beta, making it a marker of inflammation and vascular activation.\u003cbr\u003e\u003cbr\u003eIntercellular adhesion molecule 1 plays a central role in leukocyte adhesion and transmigration across endothelial barriers. By binding LFA1 on lymphocytes and neutrophils, ICAM1 facilitates firm adhesion following rolling interactions mediated by selectins. Research using ICAM1 antibody has demonstrated that blockade of this interaction reduces immune cell recruitment, underscoring its role in inflammatory responses. This pathway is particularly important in autoimmune disease, infection, and transplant rejection, where excessive leukocyte infiltration contributes to pathology.\u003cbr\u003e\u003cbr\u003eICAM1 also contributes to signaling pathways that regulate cell survival, apoptosis, and cytoskeletal rearrangements. Engagement of ICAM1 initiates intracellular signaling cascades involving kinases and actin binding proteins. Studies with ICAM1 antibody have revealed that these signals promote endothelial barrier regulation, angiogenesis, and immune synapse formation. Because of these roles, Intercellular adhesion molecule 1 is more than a passive adhesion molecule; it actively shapes cellular communication and tissue dynamics.\u003cbr\u003e\u003cbr\u003eIn oncology, ICAM1 expression is altered in many cancers. Elevated ICAM1 on tumor cells can promote immune recognition, but in other contexts it facilitates tumor progression and metastasis by enhancing interactions with immune cells and endothelial barriers. Research with ICAM1 antibody has demonstrated that ICAM1 expression correlates with prognosis in melanoma, breast, and pancreatic cancer, making it both a biomarker and potential therapeutic target. Viral pathogens such as rhinoviruses also exploit ICAM1 as a receptor for entry, further highlighting its diverse biological importance.\u003cbr\u003e\u003cbr\u003eICAM1 antibody is applied in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. Western blotting confirms expression changes under inflammatory stimulation, while immunohistochemistry highlights localization in inflamed tissues and tumors. Flow cytometry with ICAM1 antibody allows quantification of surface expression on immune and endothelial cells. These applications provide critical insight into adhesion biology and immune regulation.\u003cbr\u003e\u003cbr\u003eBy supplying validated ICAM1 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ICAM1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ICAM1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ICAM1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ICAM1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ICAM1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047309173101,"sku":"FY13020","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0139103a-0412-4c84-a0d1-c971e82a62c6.jpg?v=1782237065","url":"https:\/\/www.ebiohippo.com\/products\/icam1-antibody-cd54-bha17135922","provider":"BioHippo","version":"1.0","type":"link"}