{"product_id":"icos-cd3-zeta-nfat-reporter-lentivirus-bhv19400281","title":"ICOS-CD3ζ\/NFAT Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eICOS (inducible T cell co-stimulator) is a CD28-family co-stimulatory receptor induced on activated T cells that, upon engaging its ligand ICOS-L, enhances T cell activation, cytokine production, and helper T cell function. CD3-zeta is the principal signal-transducing subunit of the T cell receptor complex, carrying ITAM motifs that initiate activation upon antigen recognition. Fusing the ICOS ectodomain to the CD3-zeta signaling chain creates a chimeric receptor that converts ICOS-ligand engagement into a T cell receptor-like activation signal. Both ICOS co-stimulation and CD3-zeta signaling converge on NFAT, a transcription factor that drives effector gene expression, making NFAT reporters a quantitative measure of this engineered signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe ICOS-CD3ζ\/NFAT Reporter Lentivirus is a two-component immunotherapy reporter system that models co-stimulatory signaling in T cells. A receptor construct constitutively expresses the human ICOS-CD3ζ fusion protein with an antibiotic selection marker, while a reporter construct drives a dual reporter through tandem NFAT response elements. Receptor engagement activates NFAT, producing a fluorescent (GFP or RFP) and secreted Gaussia luciferase readout.\u003c\/p\u003e\n\u003cp\u003eSequential transduction and selection generate a stable, pathway-specific effector cell line. Applications include studying T cell activation, immune modulation, and co-stimulatory pathways in immunotherapy. Supplied as high-titer, VSV-G-pseudotyped lentiviral particles, the product is suitable for establishing reporter lines in primary or thawed T cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human ICOS-CD3ζ fusion under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251617030509,"sku":"TRV-0033-6S","price":895.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Vector-Layout-1024x261_167cd8ed-b44e-4b8c-9198-d933ec1f4e97.jpg?v=1782157779","url":"https:\/\/www.ebiohippo.com\/products\/icos-cd3-zeta-nfat-reporter-lentivirus-bhv19400281","provider":"BioHippo","version":"1.0","type":"link"}