{"product_id":"id3-antibody-fitc-conjugated-bha10528746","title":"ID3 Antibody, FITC conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis polyclonal anti-ID3 antibody raised in Rabbit, conjugated with FITC. Designed to detect ID3 in Human for cell biology research.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human DNA-binding protein inhibitor ID-3 protein (27-99AA) — determines epitope; confirm sample prep compatibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary matching isotype IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal:\u003c\/strong\u003e Multiple epitopes = robust signal; lot-to-lot variability — include controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Match secondary, Protein A\/G, and isotype controls to IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Protein G purification):\u003c\/strong\u003e Enriches Ig classes; reduces non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFITC:\u003c\/strong\u003e Direct fluorescence (Ex490\/Em525); protect from light; use antifade.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eID3\u003c\/strong\u003e (also: 1R21, bHLHb25, Class B basic helix-loop-helix protein 25, DNA binding protein inhibitor ID 3, DNA-binding protein inhibitor ID-3, HEIR 1, HEIR1, Helix loop helix protein HEIR 1, Helix loop helix protein HEIR1, Helix-loop-helix protein HEIR-1, HLH642, ID 3, ID like protein inhibitor HLH 1R21, ID like protein inhibitor HLH 462, ID-like protein inhibitor HLH 1R21, ID3, ID3_HUMAN, Inhibitor of DNA binding 3, Inhibitor of DNA binding 3 dominant negative helix loop helix protein) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current ID3 annotation relevant to cell biology.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003eRefer to product documentation for confirmed application data.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e IgG from Rabbit at equivalent concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal: validate with KO\/KD controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Verify dilution linearity in your sample matrix.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Confirmed for Human; extrapolation requires validation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\u003c!-- Sources: UniProt, NCBI Gene, Cusabio, Human Protein Atlas, PubMed --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53240835309933,"sku":"CSB-PA01959C0Rb-50UG","price":170.98,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53240938135917,"sku":"CSB-PA01959C0Rb-100UG","price":307.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Cusabio_Antibody_Placeholder_479f0a01-be6a-4689-aafe-e245b84daa85.jpg?v=1784926912","url":"https:\/\/www.ebiohippo.com\/products\/id3-antibody-fitc-conjugated-bha10528746","provider":"BioHippo","version":"1.0","type":"link"}