{"product_id":"idh3b-antibody-isocitric-dehydrogenase-subunit-beta-bha17135911","title":"IDH3B Antibody \/ Isocitric dehydrogenase subunit beta","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eIDH3B Antibody \/ Isocitric dehydrogenase subunit beta is a anti-IDH3B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e IDH3B\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IP, IHC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eIDH3B antibody detects Isocitrate dehydrogenase [NAD]-subunit beta, a mitochondrial enzyme subunit that participates in the tricarboxylic acid (TCA) cycle. The UniProt recommended name is Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial (IDH3B). This enzyme catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADH that fuels ATP synthesis through oxidative phosphorylation.\u003cbr\u003e\u003cbr\u003eFunctionally, IDH3B antibody identifies a 389-amino-acid mitochondrial matrix protein that forms part of the heterotetrameric IDH3 enzyme complex, along with alpha and gamma subunits (IDH3A and IDH3G). IDH3B provides structural stability and catalytic support, maintaining proper enzyme kinetics and cofactor affinity. This enzyme's reaction is irreversible under physiological conditions and represents a key regulatory step in the TCA cycle, linking carbohydrate, lipid, and amino acid metabolism.\u003cbr\u003e\u003cbr\u003eThe IDH3B gene is located on chromosome 20p13 and encodes a protein expressed in energy-demanding tissues such as heart, skeletal muscle, and brain. IDH3B ensures efficient NADH production for ATP generation and contributes to metabolic integration between glycolysis and mitochondrial respiration. Its expression adapts to cellular energy status and oxygen availability, aligning mitochondrial metabolism with energy demand.\u003cbr\u003e\u003cbr\u003eMutations or reduced expression of IDH3B can impair mitochondrial respiration, resulting in metabolic dysfunction and neurodegenerative phenotypes. In contrast to NADP+-dependent IDH1 and IDH2, IDH3B is strictly NAD+-dependent and operates exclusively within mitochondria. Dysregulation of IDH3B expression has been observed in cancers and inherited mitochondrial disorders, where altered TCA cycle flux influences redox homeostasis and biosynthetic metabolism.\u003cbr\u003e\u003cbr\u003eIDH3B antibody is widely used in metabolism, mitochondrial biology, and enzymology research. It is suitable for western blotting, immunofluorescence, and metabolic profiling assays to detect IDH3B in tissue or cell lysates. This antibody aids in studying mitochondrial energy metabolism, redox regulation, and enzyme complex formation. In disease models, IDH3B serves as a biomarker for mitochondrial dysfunction and altered oxidative metabolism.\u003cbr\u003e\u003cbr\u003eStructurally, IDH3B forms part of the IDH3 heterotetramer, contributing to the active site environment through hydrogen-bonding and metal ion coordination. Post-translational modifications, including phosphorylation and acetylation, regulate its catalytic efficiency and protein-protein interactions.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=IDH3B - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=IDH3B - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=IDH3B - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/IDH3B - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=IDH3B+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047309566317,"sku":"FY13009","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f57a0287-0475-40c5-808a-8cd74f266032.jpg?v=1782237083","url":"https:\/\/www.ebiohippo.com\/products\/idh3b-antibody-isocitric-dehydrogenase-subunit-beta-bha17135911","provider":"BioHippo","version":"1.0","type":"link"}