{"product_id":"idnk-antibody-gluconokinase-bha17135909","title":"IDNK Antibody \/ Gluconokinase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eIDNK Antibody \/ Gluconokinase is a anti-IDNK Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e IDNK\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eIDNK antibody detects Gluconokinase, an enzyme that catalyzes the phosphorylation of D-gluconate to 6-phosphogluconate in the alternative pentose phosphate pathway. The UniProt recommended name is Gluconokinase (IDNK). This cytosolic enzyme links carbohydrate metabolism with NADPH generation and oxidative stress defense by feeding intermediates into the pentose phosphate shunt.\u003cbr\u003e\u003cbr\u003eFunctionally, IDNK antibody identifies a 314-amino-acid enzyme that catalyzes the ATP-dependent phosphorylation of gluconate. The reaction product, 6-phosphogluconate, enters the oxidative branch of the pentose phosphate pathway, contributing to NADPH production and ribose biosynthesis. These processes are vital for maintaining redox balance and supporting anabolic metabolism in proliferating cells.\u003cbr\u003e\u003cbr\u003eThe IDNK gene is located on chromosome 1p36.11 and encodes a kinase belonging to the gluconokinase family. IDNK functions primarily in tissues with active glucose metabolism, such as liver and kidney, where it provides an alternative route for pentose phosphate pathway intermediates. The enzyme's activity is regulated by substrate availability and energy status, integrating carbohydrate metabolism with cellular redox needs.\u003cbr\u003e\u003cbr\u003eIn metabolic regulation, IDNK contributes to the detoxification of gluconate derived from glucose oxidation and polyol pathway intermediates. It also plays a role in microbial defense and oxidative stress adaptation by maintaining adequate NADPH pools. Altered IDNK expression has been observed in metabolic disorders, cancer, and diabetic tissues, reflecting its role in redox homeostasis and biosynthetic metabolism.\u003cbr\u003e\u003cbr\u003eIDNK antibody is widely used in enzymology, metabolism, and redox biology research. It is suitable for immunoblotting, enzyme activity assays, and metabolic flux studies to analyze IDNK expression and function. This antibody supports investigations into pentose phosphate pathway regulation, antioxidant defense, and carbohydrate catabolism. In cancer studies, IDNK detection helps elucidate metabolic rewiring associated with elevated NADPH demand.\u003cbr\u003e\u003cbr\u003eStructurally, IDNK adopts an alpha\/beta-fold typical of sugar kinases, with conserved ATP-binding and substrate recognition motifs. Its catalytic mechanism involves a sequential binding of ATP and gluconate, followed by phosphoryl transfer.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=IDNK - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=IDNK - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=IDNK - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/IDNK - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=IDNK+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047309533549,"sku":"FY13007","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_640eab49-42f8-4058-a0fd-4d29149fe48f.jpg?v=1782237062","url":"https:\/\/www.ebiohippo.com\/products\/idnk-antibody-gluconokinase-bha17135909","provider":"BioHippo","version":"1.0","type":"link"}