{"product_id":"ier3-antibody-immediate-early-response-gene-3-bha17135834","title":"IER3 Antibody \/ Immediate early response gene 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eIER3 Antibody \/ Immediate early response gene 3 is a anti-IER3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e IER3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eIER3 antibody detects Immediate early response gene 3 protein, a stress-inducible regulator of apoptosis and cell survival pathways. The UniProt recommended name is Immediate early response 3 (IER3), also known as IEX-1, DIF-2, gly96, and radiation-inducible protein. IER3 acts as a rapid responder to stress, mitogenic, and inflammatory signals, mediating downstream responses to NF-kappaB, p53, and MAPK pathways. Its expression is transiently induced by stimuli such as UV radiation, cytokines (TNF-alpha, IL-1), and growth factors, reflecting its role in adaptive cell signaling.\u003cbr\u003e\u003cbr\u003eFunctionally, IER3 antibody targets a multifunctional protein that modulates apoptosis by interacting with BCL2 family members and regulating mitochondrial membrane potential. It can act as either pro- or anti-apoptotic depending on cell context and stimulus. IER3 has been implicated in cell cycle control, stress tolerance, and immune regulation, with overexpression protecting cells from TNF-induced apoptosis via suppression of caspase-3 activation. Conversely, downregulation promotes sensitivity to radiation and chemotherapeutic stress, linking IER3 to cancer therapy resistance mechanisms.\u003cbr\u003e\u003cbr\u003eIER3 localizes mainly to the cytoplasm but can also translocate to the nucleus in certain stress conditions. The IER3 antibody is valuable for monitoring expression patterns across tissues, including spleen, thymus, and epithelial cells, and in cancers such as leukemia, breast, and colon carcinoma. The IER3 gene is located on chromosome 6p21.33, close to the major histocompatibility complex (MHC) region, encoding a 156-amino acid protein. Its promoter contains NF-kappaB binding sites, allowing rapid induction following stress or infection. IER3 acts as a negative feedback regulator in immune responses, balancing cytokine signaling and cellular resilience.\u003cbr\u003e\u003cbr\u003eIER3 also regulates ERK and AKT phosphorylation states, controlling cellular energy metabolism and proliferation. Its modulation of mitochondrial ATP synthase interaction contributes to ROS balance. \u003cbr\u003e\u003cbr\u003eThrough its unique duality in regulating cell survival, IER3 remains a critical molecular switch in stress adaptation, immune tolerance, and oncogenesis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=IER3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=IER3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=IER3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/IER3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=IER3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047307272557,"sku":"FY12932","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7a260ff1-613b-4826-b963-7c20de446726.jpg?v=1782237081","url":"https:\/\/www.ebiohippo.com\/products\/ier3-antibody-immediate-early-response-gene-3-bha17135834","provider":"BioHippo","version":"1.0","type":"link"}