{"product_id":"ifi30-antibody-gilt-gamma-interferon-inducible-lysosomal-thiol-reductase-bha17136003","title":"IFI30 Antibody \/ GILT \/ Gamma-interferon-inducible lysosomal thiol reductase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eIFI30 Antibody \/ GILT \/ Gamma-interferon-inducible lysosomal thiol reductase is a anti-IFI30 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e IFI30\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eIFI30 antibody detects Gamma-interferon-inducible lysosomal thiol reductase, an enzyme that catalyzes the reduction of disulfide bonds in endosomal and lysosomal compartments. The UniProt recommended name is Gamma-interferon-inducible lysosomal thiol reductase (IFI30). This enzyme facilitates antigen processing by reducing disulfide-linked epitopes within endosomes, promoting peptide loading onto MHC class II molecules for immune presentation.\u003cbr\u003e\u003cbr\u003eFunctionally, IFI30 antibody identifies a 250-amino-acid glycoprotein localized to lysosomes and late endosomes. IFI30 acts in concert with gamma-interferon-induced pathways to maintain redox balance within antigen-presenting cells. Its active site cysteines participate in thiol-disulfide exchange reactions, regenerating reduced peptides that can be efficiently bound by MHC class II for recognition by CD4+ T cells.\u003cbr\u003e\u003cbr\u003eThe IFI30 gene is located on chromosome 19p13.1 and is expressed predominantly in macrophages, dendritic cells, and B lymphocytes. Its expression is upregulated by interferon-gamma signaling and inflammatory cytokines. IFI30 functions downstream of antigen uptake and proteolysis, serving as a critical component in adaptive immune activation and tolerance maintenance.\u003cbr\u003e\u003cbr\u003ePathologically, altered IFI30 activity has been linked to immune disorders and cancer. Overexpression can enhance antigen presentation and immune surveillance, while deficiencies impair immune responses and tolerance mechanisms. IFI30 is also explored as a marker of macrophage activation and tumor immunogenicity. Research using IFI30 antibody supports immunology, antigen processing, and redox regulation studies.\u003cbr\u003e\u003cbr\u003eIFI30 antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence to detect lysosomal enzymes and immune regulators. \u003cbr\u003e\u003cbr\u003eStructurally, Gamma-interferon-inducible lysosomal thiol reductase contains a thioredoxin-like domain and a signal peptide targeting it to lysosomes. The protein undergoes proteolytic maturation to its active form within acidic compartments. This antibody facilitates the study of IFI30's enzymatic and immunomodulatory roles in antigen processing and cellular redox maintenance.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=IFI30 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=IFI30 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=IFI30 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/IFI30 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=IFI30+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047312646509,"sku":"FY13101","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_cfb80a04-1f64-42cc-889e-482eac1874f4.jpg?v=1782237085","url":"https:\/\/www.ebiohippo.com\/products\/ifi30-antibody-gilt-gamma-interferon-inducible-lysosomal-thiol-reductase-bha17136003","provider":"BioHippo","version":"1.0","type":"link"}