{"product_id":"ift20-antibody-intraflagellar-transport-protein-20-homolog-bha17136146","title":"IFT20 Antibody \/ Intraflagellar transport protein 20 homolog","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eIFT20 Antibody \/ Intraflagellar transport protein 20 homolog is a anti-IFT20 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e IFT20\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eIFT20 antibody detects Intraflagellar transport protein 20 homolog, a component of the intraflagellar transport (IFT) complex required for cilium assembly, maintenance, and cargo trafficking. The UniProt recommended name is Intraflagellar transport protein 20 homolog (IFT20). This small protein serves as a crucial adaptor that links vesicular trafficking machinery to the IFT complex, ensuring proper delivery of membrane and signaling components to the cilium.\u003cbr\u003e\u003cbr\u003eFunctionally, IFT20 antibody identifies a 132-amino-acid protein localized to the Golgi apparatus and the base of cilia. IFT20 associates with the IFT-B complex and interacts with proteins such as IFT57, IFT88, and GMAP210 to coordinate transport of ciliary cargo from the Golgi to the basal body. It plays essential roles in ciliogenesis, ciliary membrane protein sorting, and Hedgehog signaling. IFT20 functions both in ciliated cells, such as renal epithelial cells and photoreceptors, and in non-ciliated cells, where it contributes to vesicle trafficking and immune synapse formation.\u003cbr\u003e\u003cbr\u003eThe IFT20 gene is located on chromosome 17q25.3 and is ubiquitously expressed in tissues with active ciliary assembly, including kidney, lung, retina, and brain. Expression is dynamically regulated during cell cycle progression and development, reflecting its importance in signaling and organelle biogenesis.\u003cbr\u003e\u003cbr\u003ePathologically, mutations or loss of IFT20 disrupt cilium formation and function, leading to a spectrum of ciliopathies such as polycystic kidney disease, retinal degeneration, and developmental disorders. Altered expression may also affect immune function, as IFT20 participates in T-cell receptor recycling. Research using IFT20 antibody supports studies in cilia biology, vesicular trafficking, and developmental signaling pathways.\u003cbr\u003e\u003cbr\u003eIFT20 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect ciliary transport proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Intraflagellar transport protein 20 homolog is a small coiled-coil protein that integrates into the IFT-B complex. Its N-terminal region anchors it to the Golgi, while the C-terminal region mediates interactions with IFT components. This structural configuration enables IFT20 to couple vesicular transport and ciliary assembly. This antibody aids in investigating IFT20's role in cellular signaling, ciliogenesis, and trafficking fidelity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=IFT20 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=IFT20 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=IFT20 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/IFT20 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=IFT20+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047318937965,"sku":"FY13244","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d7b4dda4-b0fb-468d-ab9a-7ebdad60d759.jpg?v=1782237074","url":"https:\/\/www.ebiohippo.com\/products\/ift20-antibody-intraflagellar-transport-protein-20-homolog-bha17136146","provider":"BioHippo","version":"1.0","type":"link"}