{"product_id":"il-10-secreting-dendritic-cell-line-mutu-dc1940-il-10-bhc10900247","title":"IL-10 Secreting Dendritic Cell Line (MuTu DC1940-IL-10)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe MuTu DC1940-IL-10 cell line is derived from murine tumor (MuTu) dendritic cells (DC) expressing IL-10. To generate the IL-10 DC line, IL10 gene was obtained from the cDNA of MuTuDC1s stimulated with CpG and amplified by PCR.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e IL-10 Secreting Dendritic Cell Line (MuTu DC1940-IL-10) is supplied as a tumor cell line derived from Mouse spleen.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, show dendrites\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow V (TM015) + 8% FBS + 0.00005M Beta-Mercaptoethanol (IMPORTANT cells will not grow without) + 10 mM HEPES + 1% P\/S + 0.075% of 7.5% sodium bicarbonate, 37.0°C, 5% CO₂. Must batch test FBS for compatibiltiy with cell line growth. To detach cells, incubate with cold PBS + 5 mM EDTA for one minute.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in spleen biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eDNA fragments were purified and inserted into lentiviral vectors. Transgene expression and protein production was confimed through FACS and ELISA. Cells produce IL-6 and IL12p40 when stimulated with CpG1826.This cell line is a novel stable, and functional DC line model for DC related research. Donor\/background information provided for this product: CD11c:SV40LgT-transgenic C57BL\/6 mice.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow V (TM015) + 8% FBS + 0.00005M Beta-Mercaptoethanol (IMPORTANT cells will not grow without) + 10 mM HEPES + 1% P\/S + 0.075% of 7.5% sodium bicarbonate, 37.0°C, 5% CO₂. Must batch test FBS for compatibiltiy with cell line growth. To detach cells, incubate with cold PBS + 5 mM EDTA for one minute.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/ml):\u003c\/strong\u003e 100,000 - 1,000,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180494217581,"sku":"T8053","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ugytcTlHef5iCHV7PxAXfAEKJL4mkPpbWmE24AcU.png?v=1774957737","url":"https:\/\/www.ebiohippo.com\/products\/il-10-secreting-dendritic-cell-line-mutu-dc1940-il-10-bhc10900247","provider":"BioHippo","version":"1.0","type":"link"}