{"product_id":"il10-antibody-interleukin-10-bha17135760","title":"IL10 Antibody \/ Interleukin 10","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eIL10 Antibody \/ Interleukin 10 is a anti-IL10 Rabbit antibody Recombinant Rabbit Monoclonal clone AOIG-9 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e IL10\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone AOIG-9, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eIL10 antibody detects Interleukin 10, a pleiotropic anti-inflammatory cytokine that suppresses immune activation and maintains immune homeostasis. Encoded by the IL10 gene on chromosome 1q32.1, this cytokine is secreted primarily by monocytes, T regulatory cells, macrophages, and B cells. IL10 acts by limiting the expression of proinflammatory cytokines, antigen presentation, and costimulatory molecules, thereby protecting tissues from excessive immune-mediated damage.\u003cbr\u003e\u003cbr\u003eInterleukin 10 functions through binding to the IL10 receptor complex, composed of IL10RA and IL10RB subunits, triggering activation of JAK1 and TYK2 kinases. This leads to phosphorylation of STAT3, which translocates to the nucleus to induce anti-inflammatory gene expression. Targets include SOCS3, IL1RA, and other regulatory molecules that dampen NF-?B-mediated transcription of inflammatory mediators such as TNF, IL1, and IL6. IL10 signaling is essential for balancing immune tolerance with pathogen defense.\u003cbr\u003e\u003cbr\u003eThe IL10 antibody is widely used in immunology, inflammation, and infectious disease research to study cytokine signaling, immune regulation, and autoimmune disorders. Western blot analysis detects a 19 kilodalton band corresponding to IL10, while ELISA and immunofluorescence applications reveal secretion patterns and localization in immune cell cultures. This antibody supports detailed characterization of IL10 expression in response to infection, inflammation, and therapeutic modulation.\u003cbr\u003e\u003cbr\u003eDeficiency or dysregulation of IL10 signaling leads to chronic inflammation and autoimmune pathologies, including inflammatory bowel disease, rheumatoid arthritis, and psoriasis. Conversely, elevated IL10 levels in tumors and chronic infections can suppress immune responses and promote immune evasion. Therapeutic modulation of IL10 signaling is under investigation for treating autoimmune and inflammatory diseases, highlighting its dual protective and suppressive roles. The IL10 antibody provides a reliable tool for tracking cytokine expression, validating immunomodulatory pathways, and developing anti-inflammatory therapies.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=IL10 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=IL10 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=IL10 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/IL10 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=IL10+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047303864685,"sku":"FY12858","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_1baa9cdd-74bd-48a7-bb22-7976f1b76647.jpg?v=1782237041","url":"https:\/\/www.ebiohippo.com\/products\/il10-antibody-interleukin-10-bha17135760","provider":"BioHippo","version":"1.0","type":"link"}