{"product_id":"il12a-recombinant-monoclonal-antibody-bha10575857","title":"IL12A Recombinant Monoclonal Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a monoclonal anti-IL12A antibody raised in Human, with confirmed utility in ELISA, IHC, FC. It is designed to detect IL12A protein in Human and supports researchers working in others contexts.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human IL12A protein — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species (Human):\u003c\/strong\u003e Requires anti-human-IgG secondary reagents for indirect detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMonoclonal format:\u003c\/strong\u003e Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG1):\u003c\/strong\u003e Matched secondary antibodies and isotype controls required. Compatible with standard Protein A\/G purification workflows.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Affinity chromatography):\u003c\/strong\u003e Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eIL12A\u003c\/strong\u003e (also referred to as CLMF, CLMF p35, CLMF p40, CLMF1, CLMF2, Cytotoxic lymphocyte maturation factor 1, p35, Cytotoxic lymphocyte maturation factor 2, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL 12A, IL 2 subunit p40, IL-12 subunit p35, IL-12A, IL-12B, IL12, IL12, p35 subunit, IL12, subunit p40, IL12A, IL12A_HUMAN, IL12B, IL35 subunit, Interleukin 12 alpha chain, Interleukin 12 beta chain, Interleukin 12 subunit beta, Interleukin 12, p40, Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35), Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), Interleukin 12B, Interleukin-12 subunit alpha, Natural killer cell stimulatory factor 1, 35 kD subunit, natural killer cell stimulatory factor 2, Natural killer cell stimulatory factor, 40 kD subunit, NF cell stimulatory factor chain 1, NFSK, NK cell stimulatory factor chain 1, NK cell stimulatory factor chain 2, NKSF, NKSF1, NKSF2, P35, briakinumab) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of IL12A biology in others.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIHC:\u003c\/strong\u003e Visualization of target in FFPE or frozen tissue sections. Optimize antigen retrieval and secondary detection for each tissue type.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFC:\u003c\/strong\u003e Applicable for FC-based detection; refer to product documentation for optimized conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e Use an isotype-matched (IgG1 from Human) control at equivalent concentration to assess non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Confirm the clone does not cross-react with related family members under your assay conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Confirmed for Human. Extrapolation to untested species requires empirical validation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eThis product is a recombinant monoclonal antibody expressed in HEK293F cells.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003c!-- Sources:\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio: https:\/\/www.cusabio.com\n- Human Protein Atlas: https:\/\/www.proteinatlas.org\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 uL","offer_id":53248399442285,"sku":"CSB-RA011586MA1HU-50UL","price":210.0,"currency_code":"USD","in_stock":true},{"title":"100 uL","offer_id":53248471499117,"sku":"CSB-RA011586MA1HU-100UL","price":350.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-RA011586MA1HU-IHC-1.jpg?v=1776795472","url":"https:\/\/www.ebiohippo.com\/products\/il12a-recombinant-monoclonal-antibody-bha10575857","provider":"BioHippo","version":"1.0","type":"link"}