{"product_id":"il5ra-antibody-cd125-bha17135613","title":"IL5RA Antibody \/ CD125","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eIL5RA Antibody \/ CD125 is a anti-IL5RA Rabbit antibody Recombinant Rabbit Monoclonal clone 31I14 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e IL5RA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31I14, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eIL5RA antibody detects interleukin 5 receptor alpha chain, also known as CD125, encoded by the IL5RA gene. IL5RA forms part of the receptor for interleukin 5, a cytokine that regulates eosinophil proliferation, differentiation, and survival. The receptor consists of a ligand specific alpha chain and a common beta chain shared with receptors for IL3 and GM CSF. Engagement of IL5 with IL5RA triggers JAK STAT and PI3K pathways that drive eosinophil activation and immune responses.\u003cbr\u003e\u003cbr\u003eIL5RA antibody is widely applied in immunology, allergy research, and hematology. Expression of IL5RA is restricted to eosinophils, basophils, and certain progenitor cells, making it a valuable marker of eosinophilic inflammation. By detecting IL5RA, researchers can evaluate eosinophil biology in conditions such as asthma, eosinophilic esophagitis, and hypereosinophilic syndromes.\u003cbr\u003e\u003cbr\u003e Flow cytometry with IL5RA antibody distinguishes eosinophil populations in blood and tissue. Immunohistochemistry maps receptor expression in inflamed airway or gastrointestinal biopsies, while western blotting confirms protein expression in hematopoietic samples. These techniques provide robust tools for studying IL5RA expression and signaling.\u003cbr\u003e\u003cbr\u003eIL5RA is a therapeutic target in eosinophilic diseases. Monoclonal antibodies such as benralizumab target IL5RA to deplete eosinophils by antibody dependent cytotoxicity. By applying IL5RA antibody, researchers can monitor receptor levels, assess drug effects, and explore mechanisms of eosinophil regulation.\u003cbr\u003e\u003cbr\u003eBeyond allergy and hematology, IL5RA contributes to host defense against parasites by supporting eosinophil mediated immunity. Dysregulation leads to pathological inflammation in chronic asthma and gastrointestinal disorders. IL5RA antibody therefore provides a critical reagent for understanding type 2 immune responses in health and disease.\u003cbr\u003e\u003cbr\u003eIL5RA antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=IL5RA - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=IL5RA - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=IL5RA - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/IL5RA - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=IL5RA+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047296622957,"sku":"FY12710","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c1e2a708-f860-49c7-94d2-a2ef8cd5a9ca.jpg?v=1772019331","url":"https:\/\/www.ebiohippo.com\/products\/il5ra-antibody-cd125-bha17135613","provider":"BioHippo","version":"1.0","type":"link"}