{"product_id":"immortalized-bovine-satellite-cell-line-ibsc-bhc10902232","title":"Immortalized Bovine Satellite Cell Line (iBSC)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe Immortalized Bovine Satellite Cell Line (iBSC) is a robust and renewable skeletal muscle progenitor cell line derived from Bos taurus and genetically modified to overcome replicative senescence. These cells retain key characteristics of primary bovine satellite cells, including the ability to proliferate and differentiate into myotubes under appropriate culture conditions.The iBSC line is ideal for applications in cultivated meat research, muscle biology, and agricultural biotechnology, offering a consistent and scalable platform for muscle cell studies without the limitations of primary cell lifespan or donor variability.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Bovine Satellite Cell Line (iBSC) is supplied as an immortalized cell line derived from Cow muscle.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Laminin pre-coated flasks or cell culture dish (25 µg\/cm2) is required for growth. PriGrow III (TM003) + 20% FBS (*Regular) + 1 ng\/ml FGF2 (Z101455) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate 2.5 µg\/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. Do not allow the cells to reach \u0026gt;90% confluency as it will promote differentiation. Subculture the cells once reach 80%.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in muscle biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis model supports studies in muscle biology, phenotype comparison, and assay development. It can be used to examine morphology, growth behavior, and experimental responses in cultured cells. Donor\/background information provided for this product: Female, 5 weeks, Semitendinosus.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Laminin pre-coated flasks or cell culture dish (25 µg\/cm2) is required for growth. PriGrow III (TM003) + 20% FBS (*Regular) + 1 ng\/ml FGF2 (Z101455) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate 2.5 µg\/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. Do not allow the cells to reach \u0026gt;90% confluency as it will promote differentiation. Subculture the cells once reach 80%.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 2000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180544418157,"sku":"T0587","price":566.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/WQ7UzGPahcGOJaWHIYXHISwcme13af2M9GssdP8L.png?v=1782151803","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-bovine-satellite-cell-line-ibsc-bhc10902232","provider":"BioHippo","version":"1.0","type":"link"}