{"product_id":"immortalized-canine-dermal-fibroblast-negative-ebv-bhc10900450","title":"Immortalized Canine Dermal Fibroblast - EBV","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis immortalized canine dermal fibroblast cell line is a robust and reliable model for veterinary and biomedical research. These fibroblasts, derived from canine dermal tissue, are genetically modified to proliferate indefinitely while retaining essential characteristics of primary cells.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Canine Dermal Fibroblast - EBV is supplied as an immortalized cell line derived from Dog skin.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, fibroblast\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow III (TM003) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in skin biology, phenotype comparison, and assay development studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis makes them an invaluable tool for studying skin biology, wound healing, and tissue regeneration in canines. Additionally, their use extends to drug testing, toxicology studies, and understanding genetic diseases, providing insights that are translatable to human medicine. Researchers can leverage these cells to explore cellular responses, signaling pathways, and the effects of various treatments, thereby advancing both veterinary care and comparative biomedical research. Note: Several variants of the Immortalized Canine Dermal Fibroblast Cell Line are available for purchase and are differentiated by the immortalization gene. Complete list of cell lines: Cat. T0265 - Immortalized Canine Dermal Fibroblast - SV40 Cat. T0266 - Immortalized Canine Dermal Fibroblast - hTERT Cat. T0267 - Immortalized Canine Dermal Fibroblast - Myc Cat. T0268 - Immortalized Canine Dermal Fibroblast - Ras Cat. T0274 - Immortalized Canine Dermal Fibroblast - EBV\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow III (TM003) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePopulation Doubling Time (h):\u003c\/strong\u003e 50 - 60\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 20,000 – 50,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180499001709,"sku":"T0274","price":2080.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/N7v9AwO3P7vsgHay8Cf2rU4kWAssFxCSSuQP18KD.png?v=1774957751","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-canine-dermal-fibroblast-negative-ebv-bhc10900450","provider":"BioHippo","version":"1.0","type":"link"}