{"product_id":"immortalized-feline-vena-cava-endothelial-cells-negative-sv40-and-htert-bhc10900429","title":"Immortalized Feline Vena Cava Endothelial Cells - SV40 \u0026 hTERT","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eImmortalized Feline Vena Cava Endothelial Cells - SV40 \u0026amp; hTERT are derived from primary endothelial cells isolated from the vena cava inferior of cats. The endothelium is involved in a variety of pathological processes, such as tumor invasion, atherosclerosis, arthritis, thrombosis, and vasculitis.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Feline Vena Cava Endothelial Cells - SV40 \u0026amp; hTERT is supplied as an immortalized cell line derived from Cat blood vessel.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, endothelial\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow III (TM003) + 10% FBS(Regular*) + 0.1 mg\/ml gentamycin, 1mM Sodium Pyruvate (TM057), 1% Non-Essential Amino Acids (TM068) + 50µg\/ml Endothelial Cell Growth Supplement (TM130) + 10U\/ml heparin (Sigma, H5515) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Co-transduced with non-replicating lentivirus carrying SV40LT (LV665) and hTERT (LV615)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eWith a feline model, scientists may use this model to further expand the knowledge in endothelial-related diseases in feline or can use this model to address to diseases in humans as well. The Immortalized Feline Vena Cava Endothelial Cells - SV40 \u0026amp; hTERT is a valuable source for long-term studies as the cells can be continuously cultured for experiments, and retains both the phenotype and characteristics as primary cells. Immunodetection performed for hTERT and SV40L T antigen. Donor\/background information provided for this product: Estimated 1-3 year-old, Domestic Short Hair Cat.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow III (TM003) + 10% FBS(Regular*) + 0.1 mg\/ml gentamycin, 1mM Sodium Pyruvate (TM057), 1% Non-Essential Amino Acids (TM068) + 50µg\/ml Endothelial Cell Growth Supplement (TM130) + 10U\/ml heparin (Sigma, H5515) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Co-transduced with non-replicating lentivirus carrying SV40LT (LV665) and hTERT (LV615)\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180498018669,"sku":"T0517","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/thD8Af4JWgCn94nXg84RyI7Wy8H7TfK9AhjYunxf.png?v=1774957750","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-feline-vena-cava-endothelial-cells-negative-sv40-and-htert-bhc10900429","provider":"BioHippo","version":"1.0","type":"link"}