{"product_id":"immortalized-human-corneal-epithelial-cells-hce-s-bhc10900505","title":"Immortalized Human Corneal Epithelial Cells (HCE-S)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eCorneal epithelial cells tend to be difficult to maintain in an undiffentiated state in primary culture, and successful efforts to produce an immortalized human line have mostly been limited to methods involving oncogenic transformation. Immortalized Human Corneal Epithelial Cells (HCE-S) emerged spontaneously as colonies within cultures of fibroblasts derived from cadaveric cornea tissue, which were subsequently clonally isolated and passaged to a number of duplications consistent with immortalization.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Corneal Epithelial Cells (HCE-S) is supplied as an immortalized cell line derived from Human eye.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow III (TM003) + 10% FBS (Regular*) + 1X GlutaMAX + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate Note: Cells grow in dense colonies; subculture when culture vessel appears to be 70% confluent.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in eye biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eHCE-S retains a high degree of morphological similarity to primary cultures, expresses a suite of markers associated with corneal epithelial cell function (such as ABCG2, cytokeratin 3 and PAX 6 as well as integrins α9 and β1) and exhibits responsiveness to epidermal growth factor in proliferation, migration and scratch wound assays. HCE-S represents a useful tool for the study of many aspects of corneal epithelial cell biology, including regeneration and the response to growth factors, drugs or toxicity, with the added benefit of being able to grow on uncoated substrates in the absence of media supplements commonly required for similar cell lines. Donor\/background information provided for this product: Female.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow III (TM003) + 10% FBS (Regular*) + 1X GlutaMAX + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate Note: Cells grow in dense colonies; subculture when culture vessel appears to be 70% confluent.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 30,000 – 50,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180501197165,"sku":"T0737","price":710.7,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-corneal-epithelial-cells-hce-s-bhc10900505","provider":"BioHippo","version":"1.0","type":"link"}