{"product_id":"immortalized-human-cranial-periosteal-cells-tag58-bhc10900550","title":"Immortalized Human Cranial Periosteal Cells (Tag58)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe Immortalized Human Cranial Periosteal Cell Line (TAg58) is the first human cranial periosteal cell line isolated from a healthy adult patient biopsy and immortalized by lentiviral transduction with the large SV40 T antigen. The following are key characteristics that have been observed for the Immortalized Human Cranial Periosteal Cell Line (TAg58) (Alexander et al., 2015): Maintains long-term cell proliferation and osteogenic differentiation potentialShows stronger calcium phosphate mineralization than parental primary cellsMaintains similar expression patterns for most surface antigens as parental primary cellsExpresses higher levels of stem cell surface markers, CD146, and MSCA-1 (mesenchymal stem cell antigen-1), as well as higher constitutive mRNA levels of key osteogenesis factors (e.g.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Cranial Periosteal Cells (Tag58) is supplied as a tumor cell line derived from Human bone.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow IV (TM004) + 2 mM L-glutamine (G275) + 10% FBS (Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂ *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Immortalized with lentivirus containing large SV40 T antigen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in cell fate, differentiation, and expansion studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eOsteocalcin, alkaline phosphatase, runx-2) compared to parental primary cellsThe Immortalized Human Cranial Periosteal Cell Line (TAg58) represents a suitable experimental platform for studying periosteal cell biology, bone formation, and bone regeneration. Depositor Profile: Prof. Dr. Dorothea Alexander-Friedrich, University Hospital Tübingen In Germany alone, more than 10,000 people are newly diagnosed with oral cancer every year resulting in tumor and in the most cases jaw bone resection. In order to reconstruct the resulting bone defects, an alternative to autologous bone transplantation is needed. The research laboratory of the Department of Oral and Maxillofacial Surgery of the University Hospital Tübingen focuses its research on the field of bone tissue engineering (TE). Read how their work with jaw and cranial periosteal cells translate into the generation of 3D TE-constructs and lead the way to biocompatible stem cell-based therapies. Read full profile. Characterization by osteogenic differentiation experiments, Western blot, flow cytometry, detection of cell mineralization by fluorescent osteoImage staining, and gene expression analysis. Donor\/background information provided for this product: Healthy adult patient biopsy.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow IV (TM004) + 2 mM L-glutamine (G275) + 10% FBS (Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂ *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Immortalized with lentivirus containing large SV40 T antigen\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180503327085,"sku":"T0716","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/T0716_Morphology.png?v=1774957758","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-cranial-periosteal-cells-tag58-bhc10900550","provider":"BioHippo","version":"1.0","type":"link"}