{"product_id":"immortalized-human-dopaminergic-neuronal-precursor-cells-luhmes-bhc10900541","title":"Immortalized Human Dopaminergic Neuronal Precursor Cells (LUHMES)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe Immortalized Human Dopaminergic Neuronal Precursor Cells, also known as the Lund Human Mesencephalic (LUHMES) cell line, is a subclone of the tetracycline-controlled, v-myc-overexpressing human mesencephalic-derived cell line MESC2.10. This cell line is unique in that it can be differentiated to acquire a dopaminergic neuron-like phenotype under appropriate growth conditions.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Dopaminergic Neuronal Precursor Cells (LUHMES) is supplied as an immortalized cell line derived from Human brain.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, flat dendritic processes\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Grow cells in culture vessels* pre-coated with 50 μg\/ml poly-L-ornithine (PLO) (TM062) and 1 μg\/ml fibronectin (TM059) in H₂O for at least 3 hours at 37°C. After 3-hour incubation, aspirate the coating solution, rinse vessel with sterile H₂O or PBS 3 times, and use the vessel right away.*Do not grow cells in culture vessels with surface areas equal to or less than 12.5 cm²; cells do not grow in 6-well, 24-well, 48-well, or 96-well plates. Advanced DMEM\/F12 (Gibco;12634010) + 1X N2 supplement (ThermoFisher Scientific) + 2 mM L-Glutamine (G275) + 40 ng\/ml rh FGF2 (Z101455) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Do not let media colour change to orange-yellow. Note: cells are sensitive to freeze-thaw conditions and take additional time to recover. For information regarding cell differentiation, please refer to the Differentiation Protocol PDF under the Documents Tab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in neurobiology, differentiation, and cell signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eLUHMES expresses functional dopamine transporter (DAT), vesicular monoamine transporter (VMAT-2), tyrosine hydroxylase (TH) and the neuronal form of β-III tubulin after differentiation. In addition to retaining dopaminergic neuronal-specific markers, LUHMES also exhibit electrophysiological properties, thus making this cell line a valuable neuronal model for neurodevelopmental studies, disease modelling and neuropharmacology. May take 4 to 5 days for cells to recover in culture. Donor\/background information provided for this product: 8-week-old fetal human ventral mesencephalon.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNeurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.\u003c\/li\u003e\n\u003cli\u003eCell-based assays that compare experimental perturbations across defined media and substrate conditions.\u003c\/li\u003e\n\u003cli\u003ePhenotype tracking using morphology, marker expression, or reporter output where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Grow cells in culture vessels* pre-coated with 50 μg\/ml poly-L-ornithine (PLO) (TM062) and 1 μg\/ml fibronectin (TM059) in H₂O for at least 3 hours at 37°C. After 3-hour incubation, aspirate the coating solution, rinse vessel with sterile H₂O or PBS 3 times, and use the vessel right away.*Do not grow cells in culture vessels with surface areas equal to or less than 12.5 cm²; cells do not grow in 6-well, 24-well, 48-well, or 96-well plates. Advanced DMEM\/F12 (Gibco;12634010) + 1X N2 supplement (ThermoFisher Scientific) + 2 mM L-Glutamine (G275) + 40 ng\/ml rh FGF2 (Z101455) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Do not let media colour change to orange-yellow. Note: cells are sensitive to freeze-thaw conditions and take additional time to recover. For information regarding cell differentiation, please refer to the Differentiation Protocol PDF under the Documents Tab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 50,000 - 100,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180502540653,"sku":"T0284","price":463.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/jwCcHfH8UXxKmRZJ7Y6XNii3dvaJ8Sk22RTtHniH.png?v=1774957759","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-dopaminergic-neuronal-precursor-cells-luhmes-bhc10900541","provider":"BioHippo","version":"1.0","type":"link"}