{"product_id":"immortalized-human-endoneurial-endothelial-cells-thendec-bhc10900535","title":"Immortalized Human Endoneurial Endothelial Cells (THEndEC)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eAs part of the blood-nerve barrier (BNB), the endoneurial endothelial plays a significant role in the regulation of influx and efflux of water, nutrients and xenobiotics that is needed to maintain the peripheral nerve internal microenviroment. The Immortalized Human Endoneurial Endothelial Cells (THEndEC) is a useful model for use in facilitated receptor-mediated in vitro BNB permeability and\/or kinetic studies, in addition to susceptibility to drug intoxication research as it retained specialized transporters (i.e.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Endoneurial Endothelial Cells (THEndEC) is supplied as an immortalized cell line derived from Human nerve.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, spindle-shaped\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow II (TM002) + 10% FBS(Regular*) + 1 ng\/ml rhFGF2 (Z101455) +10 U\/ml heparin (Sigma, Cat. H3149) + 50µg\/ml Endothelial Cell Growth Supplement (TM130) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in neurobiology, differentiation, and cell signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eP-gp, γ-GT, LAT-1, CRT and MCT-1) and restrictive barrier function similar to primary endoneurial endothelial cells. The THEndEC has been characterized to show specific binding to UEA-1 lectin and the ability of Dil-Ac-LDL uptake, indicative of α-L-fucose expression and specialized scavenger receptor expression, respectively. Further studies demonstrated transendothelial electrical resistance (TEER) and permeability to fluoresceinated sodium and dextran using THEndEC, making this cell line suitable for peripheral nerve permeability studies as well. Donor\/background information provided for this product: Not disclosed, Sciatic Nerve (no history of neurlogical disease).\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNeurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.\u003c\/li\u003e\n\u003cli\u003eCell-based assays that compare experimental perturbations across defined media and substrate conditions.\u003c\/li\u003e\n\u003cli\u003ePhenotype tracking using morphology, marker expression, or reporter output where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow II (TM002) + 10% FBS(Regular*) + 1 ng\/ml rhFGF2 (Z101455) +10 U\/ml heparin (Sigma, Cat. H3149) + 50µg\/ml Endothelial Cell Growth Supplement (TM130) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 20,000 - 40,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180502114669,"sku":"T0292","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/GJTfYOSa35ATmMmBqq7h1i9R3euE6c2jxRqh6cen.png?v=1774957758","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-endoneurial-endothelial-cells-thendec-bhc10900535","provider":"BioHippo","version":"1.0","type":"link"}