{"product_id":"immortalized-human-frontal-scalp-dermal-papilla-cells-f-clone-2-bhc10902240","title":"Immortalized Human Frontal Scalp Dermal Papilla Cells (F Clone 2)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eDermal papilla (DP) cells are specialized mesenchymal cells located at the base of hair follicles. They play a crucial role in hair growth and maintenance by interacting with surrounding cells, including keratinocytes and melanocytes.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Frontal Scalp Dermal Papilla Cells (F Clone 2) is supplied as an immortalized cell line derived from Human hair follicle.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, fibroblast-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) PriGrow III (TM003) + 10% HI-FBS + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. 5 µg\/ml Hygromycin B (G265), and 5 µg\/ml Geneticin\/G418 (G271) Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in cell fate, differentiation, and expansion studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eCultured DP cells have been widely utilized in hair growth and regeneration studies. However, they undergo significant morphological changes in vitro and progressively lose their characteristic features. Moreover, prolonged culturing leads to a decline in their growth rate and overall proliferative capacity. Androgenetic alopecia (AGA), commonly known as male pattern baldness, is a hereditary condition that primarily affects scalp hair. It is characterized by the progressive miniaturization of hair follicles, resulting in thinner, shorter, and less pigmented hair, ultimately leading to hair loss. Unlike DP cells in the occipital (O) region, DP cells in the frontal (F) region of individuals with AGA are androgen-dependent, specifically responding to dihydrotestosterone (DHT), a potent form of testosterone. F-region DP cells are more sensitive to DHT than O-region DP cells, making frontal hair follicles more prone to miniaturization and eventual hair loss. Until now, the availability of DP cell lines from both O and F regions of the same patients has been a long-standing challenge in hair growth research and therapeutic development. For the first time in history, abm has made these invaluable DP cell lines commercially accessible to scientists in both academia and biotech industries, offering a groundbreaking tool for studying hair follicle biology and developing novel treatments for AGA. Donor\/background information provided for this product: Male, 42, Androgenetic alopecia.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpansion and maintenance studies under defined growth conditions prior to downstream differentiation or phenotype analysis.\u003c\/li\u003e\n\u003cli\u003eComparative experiments that examine changes in morphology, marker expression, or functional readouts over time.\u003c\/li\u003e\n\u003cli\u003eAssay development in culture systems where media composition and substrate selection influence interpretation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) PriGrow III (TM003) + 10% HI-FBS + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. 5 µg\/ml Hygromycin B (G265), and 5 µg\/ml Geneticin\/G418 (G271) Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e3D Culture Conditions:\u003c\/strong\u003e Procedure Preparation (before 3D Culture): Thaw cells following \"Thawing Protocol\" and \"Growth Conditions\". Expand cells for one passage, then seed for spheroid generation (3D culture). Subculture Protocol:Aspirate the supernatant and wash cells with 1X PBS (pH 7.4) (G5000). Dissociate cells using 1–1.5 ml of Gentle Dissociation Solution (TM080). Observe the cells under a microscope to confirm detachment (typically within 2–10 minutes). Cells that are difficult to detach can be put in 37 °C for several minutes to facilitate detachment.Neutralize using an equal volume of complete growth media (must contain serum) or using Trypsin Neutralizing Solution (TM069).Transfer culture suspension into sterile conical centrifuge tube (G5500), and centrifuge at 125 x g for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.Aspirate supernatant and re-suspend the pellet with pre-warmed fresh complete growth media for 3D Culture. Spheroid Generation Protocol:Count cells and viability using cell counter or hemocytometer with Trypan Blue Stain (TM071). Proceed if cells are \u0026gt;90% viability.Seed 500 to 10,000 cells per well in SpheroWell™ 96 Well Plate (G7540), final volume maintained at 100 μl per well. Avoid cell clumps (clumps will not generate uniform spheroids). Count as Day 0. Tip: Fill the outermost wells of the plate with 1X PBS (pH 7.4) (G5000) to prevent evaporation of media during incubation.(Recommended) Centrifuge the sealed plate at 300 x g for 5 minutes.Place in the incubator set at 37 °C, 5% CO₂. Change Media:Every 2–3 days, add 100 μl of fresh media slowly along the wall of the well to avoid disrupting spheroids at the bottom.Take out 100 μl of media slowly and discard from the well.(Recommended, but optional) Centrifuge the sealed plate at 300 x g for 5 minutes. Monitor the Cells for 3D\/Spheroid Formation:Spheroid formation will appear between day 5 and 10.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180543828333,"sku":"T0873","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/9MojLJ79WlvuH8t8AEI3wcJk8ej1U5OpZzhFYm8C.png?v=1774957864","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-frontal-scalp-dermal-papilla-cells-f-clone-2-bhc10902240","provider":"BioHippo","version":"1.0","type":"link"}