{"product_id":"immortalized-human-granulosa-cell-line-hgl5-bhc10900570","title":"Immortalized Human Granulosa Cell Line (HGL5)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eHuman ovarian granulosa cells are the sites of estrogen and progesterone production, making them critical in studying steroid biosynthesis, steroid-metabolizing enzymes, particular growth factors, and gonadotropins. Immortalized Human Granulosa Cells (HGL5) exhibit qualities consistent with primary ovarian granulosa cells; this includes cell retraction in response to protein kinase-A activation, the ability to produce progesterone and estradiol, and the enzyme integral to estradiol production, P450arom.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Granulosa Cell Line (HGL5) is supplied as an immortalized cell line derived from Human ovary.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow IV (TM004) + 5% Cosmic Calf Serum (Hyclone) + 10% FBS (Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.*Do not heat-inactivateNote: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in ovary biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eAll of these characteristics make the cells an attractive model to study mechanisms relating to steroid biosynthesis and metabolism, in addition to other pathways. Donor\/background information provided for this product: Not disclosed.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow IV (TM004) + 5% Cosmic Calf Serum (Hyclone) + 10% FBS (Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.*Do not heat-inactivateNote: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 10,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180504080749,"sku":"T0650","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/NjojSggUWEMaew4jD25pHYMF2roz5G98b9YTmZVB.png?v=1774957761","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-granulosa-cell-line-hgl5-bhc10900570","provider":"BioHippo","version":"1.0","type":"link"}