{"product_id":"immortalized-human-hepatic-stellate-cell-line-lsc-1-bhc10902181","title":"Immortalized Human Hepatic stellate Cell Line (LSC-1)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe Immortalized Human Hepatic Stellate Cell Line (LSC-1) represents a novel advancement in the field of liver disease research, particularly in the study of liver fibrosis. Unique for its derivation from human liver tissue and immortalization using specific genetic modifications, LSC-1 exhibits key characteristics of activated hepatic stellate cells, including upregulated fibrogenic gene expression and increased matrix and adhesion protein levels.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Hepatic stellate Cell Line (LSC-1) is supplied as an immortalized cell line derived from Human liver.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent,\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow IX Medium (TM019) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: subculture cells with 0.05% Trypsin-EDTA *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in liver cancer biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis cell line reverts to a quiescent-like phenotype under certain 3D culture conditions, highlighting its versatility for studying the dynamic nature of hepatic stellate cells in fibrosis. Its application in various three-dimensional co-culture systems with HepaRG cells has demonstrated significant potential for investigating liver fibrosis mechanisms, drug discovery, and the development of antifibrotic therapies, providing a valuable tool for both basic and applied research in liver disease. Donor\/background information provided for this product: Female, Histologically normal liver samples from patients with liver tumor.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow IX Medium (TM019) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: subculture cells with 0.05% Trypsin-EDTA *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 10,000 -15,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180543435117,"sku":"T0429","price":1792.2,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/5M8wxasx4ikuPR6s8kIOQ9oudkQSj07tkZyK1Qf0.png?v=1782151805","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-hepatic-stellate-cell-line-lsc-1-bhc10902181","provider":"BioHippo","version":"1.0","type":"link"}