{"product_id":"immortalized-human-intervertebral-disc-cartilage-endplate-derived-stem-cells-negative-sv40-bhc10900600","title":"Immortalized Human Intervertebral Disc Cartilage Endplate-Derived Stem Cells - SV40","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eImmortalized Human Intervertebral Disc Cartilage Endplate-derived Stem Cells -SV40 is a valuable and reliable source for studies relating to the cartilaginous endplate and nutrient transportation in the body. The cartilage is positioned between the vertebral endplate in the spine and the nucleus pulposus in which it functions as a barrier.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Intervertebral Disc Cartilage Endplate-Derived Stem Cells - SV40 is supplied as an immortalized cell line derived from Human skeletal muscle.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal shaped\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow IV (TM004) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂ *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Serial passaging and transduction with recombinant lentiviruses carrying SV40 antigen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in cell fate, differentiation, and expansion studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eIt has a role in the health aspects of the disc such as growth and degeneration. With an immortalized cell line, researchers do not need to constantly look for donors to isolate the cells. These cells can be proliferated in vitro for downstream experiments. Donor\/background information provided for this product: Not disclosed.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpansion and maintenance studies under defined growth conditions prior to downstream differentiation or phenotype analysis.\u003c\/li\u003e\n\u003cli\u003eComparative experiments that examine changes in morphology, marker expression, or functional readouts over time.\u003c\/li\u003e\n\u003cli\u003eAssay development in culture systems where media composition and substrate selection influence interpretation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow IV (TM004) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂ *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Serial passaging and transduction with recombinant lentiviruses carrying SV40 antigen\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180503949677,"sku":"T0009","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/t0009_immortalized_human_intervertebral_disc_cartilage_endplate-derived_stem_cells_-sv40.jpg?v=1774957761","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-intervertebral-disc-cartilage-endplate-derived-stem-cells-negative-sv40-bhc10900600","provider":"BioHippo","version":"1.0","type":"link"}