{"product_id":"immortalized-human-mammary-epithelial-cells-hmec-2-6-bhc10900585","title":"Immortalized Human Mammary Epithelial Cells (HMEC 2.6)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eImmortalized Human Mammary Epithelial Cells (HMEC 2.6) is an immortalized breast epithelial cell line which is non-tumorigenic and does not show anchorage-independent growth. There is a normal distribution of cells in G1, S, and G2\/M phase and they express normal breast epithelial markers (E-cadherin, CK7\/18, and CK5\/14).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Mammary Epithelial Cells (HMEC 2.6) is supplied as an immortalized cell line derived from Human mammary.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow IV (TM004) + 1% FBS* (or 35 µg\/ml bovine pituitary extract (Hammond)) + 12.5 ng rhEGF (Z100139) + 50 µM fresh ascorbic acid + 2 nM estradiol + 1 µg\/ml insulin (TM053) + 2.8 µM hydrocortisone + 0.1 mM ethanolamine + 0.1 mM L-glutamine (G275) + 15 nM sodium selenite + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in cancer biology, phenotype comparison, and response profiling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThe cells retain primary characteristics and are valuable for research relating to mammary epithelial biology. Donor\/background information provided for this product: Female.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow IV (TM004) + 1% FBS* (or 35 µg\/ml bovine pituitary extract (Hammond)) + 12.5 ng rhEGF (Z100139) + 50 µM fresh ascorbic acid + 2 nM estradiol + 1 µg\/ml insulin (TM053) + 2.8 µM hydrocortisone + 0.1 mM ethanolamine + 0.1 mM L-glutamine (G275) + 15 nM sodium selenite + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 25,000 - 30,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180503228781,"sku":"T0454","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LhwsL9fAZkojDd9SK6rURh7fZgF9F4H1Wc5wlQYM.png?v=1774957762","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-mammary-epithelial-cells-hmec-2-6-bhc10900585","provider":"BioHippo","version":"1.0","type":"link"}