{"product_id":"immortalized-human-mammary-epithelial-progenitor-k5-positive-k19-positive-cells-negative-htert-bhc10900583","title":"Immortalized Human Mammary Epithelial Progenitor (K5+\/K19+) Cells - hTERT","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe immortalized human mammary progenitor cells – hTERT (K5+\/K19+) is a clonal cell population of progenitors coexpressing normal mammary and stem cell markers. It has the ability to self-renew and differentiate into luminal and\/or myoepithelial cell lineages.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Mammary Epithelial Progenitor (K5+\/K19+) Cells - hTERT is supplied as a tumor cell line derived from Human mammary.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, cobblestone | Spindle-shaped if cultured in mammary epithelial growth media\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). Optimized media to express basal, luminal, and stem cell markers with some myoepithelial cell markers (a-SMA, CD10, Thy-1), grow cells in DFCI-1 media. To make DFCI-1 the base medium is Prigrow VIII and Prigrow IV (1:1, vol\/vol) medium available at abm (TM018 and TM004). To make the complete growth medium, add the following components to the base medium: 1% FBS*, 1% Penicillin\/Streptomycin Solution (G255) , 10 mM HEPES (Gibco), 12.5 ng\/ml Recombinant Human EGF (Z100139), 6.5 ng\/ml triiodothyronine (Sigma), 0.545 ng\/ml beta-estradiol (Sigma), 1 µg\/ml insulin (TM053), 1 µg\/ml hydrocortisone (Sigma), 0.006X ethanolamine (Sigma), 14.1 µg\/ml phosphoethanolamine (Sigma), 10 µg\/ml transferrin (Sigma), 2 mM L-glutamine (G275), 2.6 ng\/ml sodium selenite (Sigma), 1 ng\/ml cholera toxin (Sigma), 35 µg\/ml bovine pituitary extract (Hammond Cell Tech), and freshly added 10 µg\/ml ascorbic acid (Sigma). Carbon dioxide (CO2): 5%, Temperature: 37.0°C. Change media every 2-3 days. *Do not use heat-inactivated FBS for cell culture unless specified otherwise. For cells to adopt spindle-shape morphology around tight epithelial cell colonies and for the presence of mucin-1 positive cells: grow cells in mammary epithelial growth medium (MEGM; Lonza) supplemented with B27 (10ml\/500ml medium), 20 ng\/ml Recombinant Human EGF (Z100139), 20 ng\/ml Recombinant Human FGF2 (Z101455), 4 µg\/ml heparin, and 1% Penicillin\/Streptomycin Solution (G255). Carbon dioxide (CO2): 6.5%, Temperature: 37.0°C. For information regarding cell differentiation, please refer to the Differentiation Protocol PDF under the Documents Tab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in cancer biology, phenotype comparison, and response profiling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThrough propagation in different optimized media, the cells are able to give rise to new population of cells with different morphology and characteristics, such as mucin-1 positive cells, vimentin-negative cells, or expressing basal, luminal, and other stem cell markers. Aldehyde dehydrogenase 1A3 enzyme is noticeably higher in expression, a marker commonly used for isolating normal and tumor mammary stem cells. K5+\/K19+ cells have Wnt, Notch, and hedeghog gene regulatory pathways. It is a valuable tool in research in the field of biology, the stem\/progenitor origin, and the heterogeneity mechanisms in breast cancer.Western blot was used to assess cell lineage-related and stem cell markers. Donor\/background information provided for this product: Female.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). Optimized media to express basal, luminal, and stem cell markers with some myoepithelial cell markers (a-SMA, CD10, Thy-1), grow cells in DFCI-1 media. To make DFCI-1 the base medium is Prigrow VIII and Prigrow IV (1:1, vol\/vol) medium available at abm (TM018 and TM004). To make the complete growth medium, add the following components to the base medium: 1% FBS*, 1% Penicillin\/Streptomycin Solution (G255) , 10 mM HEPES (Gibco), 12.5 ng\/ml Recombinant Human EGF (Z100139), 6.5 ng\/ml triiodothyronine (Sigma), 0.545 ng\/ml beta-estradiol (Sigma), 1 µg\/ml insulin (TM053), 1 µg\/ml hydrocortisone (Sigma), 0.006X ethanolamine (Sigma), 14.1 µg\/ml phosphoethanolamine (Sigma), 10 µg\/ml transferrin (Sigma), 2 mM L-glutamine (G275), 2.6 ng\/ml sodium selenite (Sigma), 1 ng\/ml cholera toxin (Sigma), 35 µg\/ml bovine pituitary extract (Hammond Cell Tech), and freshly added 10 µg\/ml ascorbic acid (Sigma). Carbon dioxide (CO2): 5%, Temperature: 37.0°C. Change media every 2-3 days. *Do not use heat-inactivated FBS for cell culture unless specified otherwise. For cells to adopt spindle-shape morphology around tight epithelial cell colonies and for the presence of mucin-1 positive cells: grow cells in mammary epithelial growth medium (MEGM; Lonza) supplemented with B27 (10ml\/500ml medium), 20 ng\/ml Recombinant Human EGF (Z100139), 20 ng\/ml Recombinant Human FGF2 (Z101455), 4 µg\/ml heparin, and 1% Penicillin\/Streptomycin Solution (G255). Carbon dioxide (CO2): 6.5%, Temperature: 37.0°C. For information regarding cell differentiation, please refer to the Differentiation Protocol PDF under the Documents Tab.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 35,000 - 45,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180503621997,"sku":"T0452","price":0.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-mammary-epithelial-progenitor-k5-positive-k19-positive-cells-negative-htert-bhc10900583","provider":"BioHippo","version":"1.0","type":"link"}