{"product_id":"immortalized-human-mast-cells-luva-bhc10900579","title":"Immortalized Human Mast Cells (LUVA) ; \u003cspan style=\"color: #ef6331;\"\u003eNot capable of histamine release (degranulation)\u003c\/span\u003e","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eLUVA cell line is derived from CD34+-enriched mononuclear cells derived from a donor with aspirin exacerbated respiratory disease were cultured for 8 weeks with stem cell factor and interleukin-6 and with interleukin-3 for the first week only.LUVA cells possess metachromatic cytoplasmic granules that are immunoreactive for tryptase, cathepsin G, and carboxypeptidase A3. They express transcripts encoding genes for FcεRI, c-kit, chymase, tryptase, histidine decarboxylase, carboxypeptidase A3, and the type 1 receptor for cysteinyl leukotrienes.LUVA cells are an immortalized human mast cell line that can be maintained without stem cell factor and display high levels of normally signaling c-kit and FcεRI.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Mast Cells (LUVA) ; \u003cspan style=\"color: #ef6331;\"\u003eNot capable of histamine release (degranulation)\u003c\/span\u003e is supplied as an immortalized cell line derived from Human blood.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Suspension, round (cells may attach in non-upright culture vessel)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. PriGrow X Series Medium (TM0843) + 2 mM L-glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: We highly recommend using SpheroWell™. Grow cells in upright culture vessels if usign PriCoat. Do not culture\/freeze-down cells with FBS.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThese cells will prove valuable for functional human mast cell studies. However, immunohistochemical staining showed that only 5% cells are positive for FcεRI receptor, and thus a modest histamine release capability as compared to well-established LAD2 cell line. Note: degranulation is not a dominant feature of this cell line. Donor\/background information provided for this product: CD34+-enriched mononuclear cells derived from the peripheral blood of a donor with aspirin exacerbated respiratory disease.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. PriGrow X Series Medium (TM0843) + 2 mM L-glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: We highly recommend using SpheroWell™. Grow cells in upright culture vessels if usign PriCoat. Do not culture\/freeze-down cells with FBS.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:2 - 1:20; 1:10 split is confluent in 3-4 days.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180502999405,"sku":"T0843","price":968.2,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/q7gKId8FrOYUpEjkwk65x1097qybvH9ydEhtRzXM.png?v=1774957765","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-mast-cells-luva-bhc10900579","provider":"BioHippo","version":"1.0","type":"link"}