{"product_id":"immortalized-human-middle-ear-epithelial-cell-line-rom2-bhc10902215","title":"Immortalized Human Middle Ear Epithelial Cell Line (ROM2)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThese immortalized middle ear cell lines (Cat. T0412 - T0146) are derived from donors with cochlear implants (T0412), recurrent otitis media (T0413, T0414) and otitis media with effusion (T0415, T0416).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Immortalized Human Middle Ear Epithelial Cell Line (ROM2) is supplied as an immortalized cell line derived from Human ear.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, cobble-stone polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM0412) + 0.48 µg\/ml Hydrocortisone (TM066) + 1% Penicillin\/Streptomycin Solution (G255), 33.0°C, 5% CO₂. Change media every 2-3 days.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in ear biology, phenotype comparison, and assay development studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003ePCR were used for marker detection and cells were validated by cytogenetic analysis. These cell lines are suitable research models for investigating the molecular pathogenesis of middle ear diseases including otitis media. Complete list of cell lines in this panel: Cat. T0412 - Immortalized Human Middle Ear Epithelial Cell Line (PCI)Cat. T0413 - Immortalized Human Middle Ear Epithelial Cell Line (ROM1)Cat. T0414 - Immortalized Human Middle Ear Epithelial Cell Line (ROM2)Cat. T0415 - Immortalized Human Middle Ear Epithelial Cell Line (OME1)Cat. T0416 - Immortalized Human Middle Ear Epithelial Cell Line (OME2)\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.\u003c\/li\u003e\n\u003cli\u003eInterpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM0412) + 0.48 µg\/ml Hydrocortisone (TM066) + 1% Penicillin\/Streptomycin Solution (G255), 33.0°C, 5% CO₂. Change media every 2-3 days.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 13,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180544156013,"sku":"T0414","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/UsGNtVgJHn4HEwLlaaqOEby9C6M8ScXeScRPhoV6.jpg?v=1774957863","url":"https:\/\/www.ebiohippo.com\/products\/immortalized-human-middle-ear-epithelial-cell-line-rom2-bhc10902215","provider":"BioHippo","version":"1.0","type":"link"}